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Literature summary for 2.7.1.11 extracted from
- The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: Kinetic and structural analysis of the allosteric ATP inhibition (2011), J. Biol. Chem., 286, 5774-5783.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Escherichia coli |  |
| GDP | - |
Escherichia coli | |
| MgATP2- | the apparent Km for MgATP2- is 0.022 mM | Escherichia coli | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isozyme Pfk-2 in complex with D-fructose 6-phosphate, hanging drop vapor diffusion method, using 23% (w/v) PEG 4000, 100 mM sodium acetate, pH 4.75, and 200 mM ammonium acetate | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 6-phosphate | inhibition occurs at low concentrations of fructose 6-phosphate | Escherichia coli | |
| MgATP2- | inhibition occurs at high concentrations of MgATP2- | Escherichia coli | |
| phosphoenolpyruvate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, bound to ATP | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P06999 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | x-ray crystallography | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pfk-2 | isoform | Escherichia coli |
| phosphofructokinase-2 | isoform | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 44 | - |
D-fructose 6-phosphate | in the presence of 0.5 mM MgATP2-, at pH 8.2 and 25°C | Escherichia coli | |
| 60 | - |
D-fructose 6-phosphate | in the presence of 0.1 mM MgATP2-, at pH 8.2 and 25°C | Escherichia coli | |
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