Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli RL257 cells | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, and 18% (w/v) polyethylene glycol 8000, at 16°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains Ca2+ ions | Geobacillus stearothermophilus | |
Mg2+ | required | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P00512 | - |
- |
Purification (Comment) | Organism |
---|---|
Mimetic Blue 1 column chromatography and Mono Q column chromatography | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
PFK | - |
Geobacillus stearothermophilus |
phosphofructokinase | - |
Geobacillus stearothermophilus |