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Literature summary for 2.7.1.11 extracted from

  • Martinez-Costa, O.; Sanchez, V.; Lázaro, A.; Hernandez, E.; Tornheim, K.; Aragon, J.
    Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase (2012), Biochem. J., 445, 213-218.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate
-
 Homo sapiens
additional information not activated by D-fructose 2,6-bisphosphate Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in pfk-deficient Saccharomyces cerevisiae strain HD152-1D Homo sapiens
expressed in pfk-deficient Saccharomyces cerevisiae strain HD152-1D Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Homo sapiens
additional information not inhibited by ATP Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 ATP wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C Dictyostelium discoideum
0.07
-
 ATP wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Homo sapiens
Mg2+ required for activity Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10% (w/v) PEG precipitation, DE52 column chromatography, Blue-Sepharose column chromatography, phosphocellulose column chromatography, and Superdex 200 gel filtration Homo sapiens
10% (w/v) PEG precipitation, DE52 column chromatography, Blue-Sepharose column chromatography, phosphocellulose column chromatography, and Superdex 200 gel filtration Dictyostelium discoideum

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Homo sapiens
-
muscle
-
 Dictyostelium discoideum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Dictyostelium discoideum ADP + D-fructose 1,6-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphofructo-1-kinase
-
 Homo sapiens
6-phosphofructo-1-kinase
-
 Dictyostelium discoideum
PFK
-
 Homo sapiens
PFK
-
 Dictyostelium discoideum
phosphofructokinase
-
 Homo sapiens
phosphofructokinase
-
 Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
236
-
 ATP wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C Dictyostelium discoideum
357
-
 ATP wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3
-
 ATP wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C Homo sapiens