Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | converts saturation curves for D-fructose 6-phosphate to hyperbolic and activates PFK synergistically with AMP | Sparus aurata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | about 70% inhibition at 7 mM | Sparus aurata | |
ADP | - |
Sparus aurata | |
ATP | complete inhibition at 10 mM | Sparus aurata | |
citrate | almost complete inhibition at 1 mM citrate in the absence of D-fructose 2,6-bisphosphate | Sparus aurata | |
additional information | not inhibited by 6-phosphogluconate and phosphocreatine | Sparus aurata | |
phosphoenolpyruvate | about 30% inhibition at 5 mM | Sparus aurata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
ATP | purified enzyme, at pH 7.0, in the absence of D-fructose 2,6-bisphosphate | Sparus aurata | |
0.076 | - |
ATP | purified enzyme, at pH 7.0, in the presence of 0.005 mM D-fructose 2,6-bisphosphate | Sparus aurata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
6 * 86000, apparent molecular weight estimated from SDS-PAGE | Sparus aurata |
510000 | - |
native enzyme, gel filtration | Sparus aurata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sparus aurata | - |
gilthead sea bream | - |
Purification (Comment) | Organism |
---|---|
12% PEG6000 precipitation, ATP agarose column chromatography, DEAE-Sepharose column chromatography, phosphocellulose column chromatography, and Blue-Sepharose column chromatography | Sparus aurata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sparus aurata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
crude extract | Sparus aurata |
627 | - |
after 15700fold purification | Sparus aurata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Sparus aurata | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 86000, apparent molecular weight estimated from SDS-PAGE | Sparus aurata |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-1-kinase | - |
Sparus aurata |
PFK | - |
Sparus aurata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sparus aurata |