Application | Comment | Organism |
---|---|---|
biotechnology | design of strains with improved antibiotic production | Streptomyces coelicolor |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PFKA1-deletion strain, constructed by replacement of a chromosomal sequence within an Streptomyces coelicolor cosmid | Streptomyces coelicolor |
additional information | PFKA2-deletion strain, constructed by replacement of a chromosomal sequence in a Streptomyces coelicolor cosmid, 2-6fold increased antibiotic production, accumulation of glucose 6-phosphate, changes of intracellular fluxes, no enzyme activity | Streptomyces coelicolor |
additional information | PFKA3-deletion strain, constructed by replacement of a chromosomal sequence in a Streptomyces coelicolor cosmid | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Streptomyces coelicolor | - |
ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | O08333 | A3(2), strain M145 | - |
Streptomyces coelicolor | Q9FC99 | pfkA3 gene; A3(2), strain M145 | - |
Streptomyces coelicolor | Q9L1L8 | A3(2), strain M145 | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
characterization of the PFKA2-deletion strain by metabolic flux analysis and transcription analysis, transcriptional changes in regulatory and membrane proteins as well as in fatty acid metabolism, carbon flux through the pentose phosphate pathway increased, PFKA2 protein involved in determining the carbon flux distribution, production of the pigmented antibiotics actinorhodin and undecylprodigiosin up to 6fold increased in the deletion strain | Streptomyces coelicolor |
additional information | - |
integrated analysis of gene expression data by genome-scale metabolic model simulations, deletion mutant strain produces antibiotics in quantities comparable with that of the reference strain, enzyme activities like in wild-type attributed to intact copy of pfkA2 gene | Streptomyces coelicolor |
additional information | - |
integrated analysis of gene expression data by genome-scale metabolic model simulations, deletion mutant strain produces antibiotics in quantities comparable with that of the reference strain, wild type-like phosphofructokinase activities measured, attributed to intact copy of pfkA2 gene | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Streptomyces coelicolor | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | engineering to increase antibiotic production | Streptomyces coelicolor | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | improved antibiotic production by deletion of PFKA2, involvement of PFKA2 in determining the carbon flux distribution | Streptomyces coelicolor | ADP + D-fructose 1,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFKA1 | - |
Streptomyces coelicolor |
PFKA2 | - |
Streptomyces coelicolor |
PFKA3 | - |
Streptomyces coelicolor |