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Literature summary for 2.7.1.11 extracted from

  • Bar, J.; Schellenberger, W.; Kopperschlager, G.
    Purification and characterization of phosphofructokinase from the yeast Kluyveromyces lactis (1997), Yeast, 13, 1309-1317.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
AMP 2-5 mM, maximal activation at 3 mM ATP Kluyveromyces lactis
D-fructose 2,6-bisphosphate approx. 200-400 mM, maximal activation at 3 mM ATP Kluyveromyces lactis

Inhibitors

Inhibitors Comment Organism Structure
ATP in the absence of AMP and fructose 2,6-bisphosphate Kluyveromyces lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 D-fructose 6-phosphate pH 7.15, 25°C, in the presence of 0.1 mM fructose 2,6-bisphosphate Kluyveromyces lactis
0.37
-
 D-fructose 6-phosphate pH 7.15, 25°C, in the presence of 1 mM AMP Kluyveromyces lactis
1.58
-
 D-fructose 6-phosphate pH 7.15, 25°C Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
 x * 119000 + x * 102000, encoded by KLPFK1 and KLPFK2 genes, SDS-PAGE Kluyveromyces lactis
119000
-
 x * 119000 + x * 102000, encoded by KLPFK1 and KLPFK2 genes, SDS-PAGE Kluyveromyces lactis
845000
-
 sedimentation equilibrium analysis Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Kluyveromyces lactis
-
 ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol, Cibacron blue F 3G, Sepharose 6B, Resource Q, BioSil SEC 400 Kluyveromyces lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101
-
-
 Kluyveromyces lactis

Storage Stability

Storage Stability Organism
4°C, precipitated in 50 mM potassium phosphate, pH 7.0, 5 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM PMSF, several weeks, no loss of activity Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Kluyveromyces lactis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate no cooperativity with respect to fructose 6-phosphate Kluyveromyces lactis ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 119000 + x * 102000, encoded by KLPFK1 and KLPFK2 genes, SDS-PAGE Kluyveromyces lactis