Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
concentrated PFK solution is mixed with solutions containing fructose 6-phosphate, ADP and 2-methyl-2,4-pentanediol in a glass tube to a final concentration of 4 mg/ml PFK, 50% 2-methyl-2,4-pentanediol, 0.2 mM fructose 6-phosphate, 5 or 10 mM ATP, 100 mM Tris-HCl, pH 8.3, 5 mM MgCl2, 2.4 mM dithiothreitol, and 2.4 mM EDTA, crystal structure of PFK complexed with fructose 1,6-bisphosphate, ATP/Mg2+ and ADP/Mg2+ at 2.4 A resolution | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli | |
Mg2+ | required for activity | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing overproducing plasmid pHE1007 | - |
Escherichia coli | - |
containing overproducing plasmid pHE1012 | - |
Escherichia coli DF1020 | - |
containing overproducing plasmid pHE1007 | - |
Escherichia coli DF1020 | - |
containing overproducing plasmid pHE1012 | - |
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpressed PFK, DEAE-cellulose, Cibacron blue, hydroxyapatite | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Escherichia coli DF1020 | ADP + D-fructose 1,6-bisphosphate | - |
? |