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Literature summary for 2.7.1.11 extracted from

  • Kemerer, V.F.; Griffin, C.C.; Brand, L.
    Phosphofructokinase from Escherichia coli (1975), Methods Enzymol., 42C, 91-98.
No PubMed abstract available
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
additional information
-
 Escherichia coli
NH4+ can replace K+ Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Escherichia coli
additional information not inhibited by fructose 1,6-bisphosphate; not inhibited by pyruvate; not inhibited by ribulose 5-phosphate Escherichia coli
phosphoenolpyruvate feedback inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties of dansylated enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ can replace NH4+ Escherichia coli
Mg2+ required for activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
141000 142000
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, acetone, QAE-Sephadex, acid, QAE-Sephadex, hydroxyapatite Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
263
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate no activity with hexitol 6-phosphates Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
dATP + D-fructose 6-phosphate as good as ATP Escherichia coli dADP + D-fructose 1,6-bisphosphate
-
 ?