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Literature summary for 2.7.1.11 extracted from

  • Hofmann, E.; Kopperschläger, G.
    Phosphofructokinase from yeast (1982), Methods Enzymol., 90, 49-60.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP activation Saccharomyces cerevisiae
ADP slight Saccharomyces cerevisiae
D-fructose 2,6-bisphosphate strong positive allosteric action on PFK Saccharomyces cerevisiae
NH4+ increases maximum activity of PFK and the affinity of PFK to fructose 6-phosphate Saccharomyces cerevisiae
phosphate synergistic with AMP Saccharomyces cerevisiae

General Stability

General Stability Organism
dilution inactivates Saccharomyces cerevisiae
dilution leads to enzyme dissociation Saccharomyces cerevisiae
PMSF stabilizes during purification Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Saccharomyces cerevisiae
citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Saccharomyces cerevisiae
MgATP2-
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information pH-dependence of kinetic properties of cytosolic and plastid isozymes Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Saccharomyces cerevisiae
Mg2+ required for activity Saccharomyces cerevisiae
Mg2+ MgATP is the active substrate Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae
118000
-
alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae
835000
-
sedimentation equilibrium analysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Saccharomyces cerevisiae dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations ADP + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
baker's yeast, preferred source: pitching yeast
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Cibacron Blue F3G-A, gel filtration, ion-exchange Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate mechanism Saccharomyces cerevisiae
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate structure-oriented allosteric four-state model describing substrate and effector actions on PFK Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
0°C, dilute enzyme solution in 5 mM imidazole-HCl buffer, pH 7, 1 mM D-fructose 6-phosphate, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 6 h, 20% loss of activity Saccharomyces cerevisiae
4°C, concentrated enzyme solution, 50 mM potassium phosphate buffer, pH 7, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 1 mM fructose 6-phosphate, several months Saccharomyces cerevisiae
room temperature, 20% loss of activity within 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
octamer alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-dependence of kinetic properties Saccharomyces cerevisiae
7
-
optimum depends on the ATP and fructose 6-phosphate concentration Saccharomyces cerevisiae