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Literature summary for 2.7.1.11 extracted from
- Phosphofructokinase from Ehrlich ascites tumor (1982), Methods Enzymol., 90, 35-38.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | allosteric enzyme | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition | Mus musculus |  |
| K+ | at high concentrations, ATP reverses | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP | Mus musculus | |
| Mg2+ | required for activity | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephadex, Sephadex G-200 | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate | allosteric regulated enzyme, ping-pong bi-bi mechanism or according to Sumi and Ui, allosteric ping-pong II mechanism | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, in concentrated ammonium sulfate suspension, in the presence of ATP, at least 1 month, no loss of activity | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Mus musculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 28 | assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
ATP-independent, K+ or SO42- slightly shift to alkaline | Mus musculus |
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Release 2023.1 (January 2023)
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