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Literature summary for 2.7.1.11 extracted from

  • Leite, A.; Neto, J.A.; Leyton, J.F.; Crivellaro, O.; El-Dorry, H.A.
    Phosphofructokinase from bumblebee flight muscle. Molecular and catalytic properties and role of the enzyme in regulation of the fructose 6-phosphate/fructose 1,6-bisphosphate cycle (1988), J. Biol. Chem., 263, 17527-17533.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
AMP activation Bombus atratus
AMP synergistic with fructose 2,6-bisphosphate Bombus atratus
D-fructose 2,6-bisphosphate synergistic with AMP Bombus atratus
D-fructose 2,6-bisphosphate 3fold activation in the presence of approx. 0.003 mM, i.e. the physiological fructose 2,6-bisphosphate concentration Bombus atratus

Inhibitors

Inhibitors Comment Organism Structure
ATP at high concentrations Bombus atratus
Ca2+
-
 Bombus atratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Bombus atratus
0.039
-
 ATP pH 8.0, 30°C Bombus atratus
0.039
-
 fructose 6-phosphate pH 8.0, 30°C Bombus atratus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Bombus atratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
 glycerol density gradient centrifugation Bombus atratus

Organism

Organism UniProt Comment Textmining
Bombus atratus
-
 bumblebee
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Blue-Sepharose, density gradient centrifugation Bombus atratus

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate probably a random or compulsory-order ternary complex mechanism at pH 8.0 Bombus atratus
a

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Bombus atratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
123
-
-
 Bombus atratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Bombus atratus ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer SDS-PAGE Bombus atratus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bombus atratus