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Literature summary for 2.7.1.11 extracted from

  • Starling, J.A.; Allen, B.L.; Kaeini, M.R.; Payne, D.M.; Blytt, H.J.; Hofer, H.W.; Harris, B.G.
    Phosphofructokinase from Ascaris suum. Purification and properties (1982), J. Biol. Chem., 257, 3795-3800.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
-
Ascaris suum
360000
-
gel filtration at high ionic strength, in 50 mM Tris-phosphate buffer plus 5 mM dithiothreitol, the enzyme aggregates to higher molecular weight form of 520000 Da Oryctolagus cuniculus
398000
-
-
Oryctolagus cuniculus
398000
-
gel filtration at high ionic strength, in 50 mM Tris-phosphate buffer plus 5 mM dithiothreitol, the enzyme aggregates to higher molecular weight forms of 520000 Da and 660000 Da Ascaris suum

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
female parasitic nematode, from small intestine of pig
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, phosphocellulose, ammonium sulfate Ascaris suum

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Oryctolagus cuniculus
-
muscle
-
Oryctolagus cuniculus
-
muscle
-
Ascaris suum
-

Storage Stability

Storage Stability Organism
4°C, under nitrogen, in 50 mM Tris-potassium phosphate buffer, pH 8, 1 mM EDTA, 1.4 M (NH4)2SO4, 1 mM ATP, 0.2 M KCl, 10 mM dithiothreitol, at least 6 months Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Ascaris suum ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 94600-95000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ascaris suum