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Literature summary for 2.7.1.11 extracted from
- Molecular, kinetic, and immunological properties of the 6-phosphofructokinase from the green alga Selenastrum minutum (1990), Plant Physiol., 93, 871-879.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | 25% activation at 5 mM, inhibition above | Monoraphidium minutum |  |
General Stability
| General Stability | Organism |
|---|---|
| at least 15 mM phosphate required to stabilize PFK during purification, presence of 10% glycerol helps to stabilize | Monoraphidium minutum |
| largest molecular weight-form of purified enzyme is the most stable form | Monoraphidium minutum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | 2 mM, 92% inhibition | Monoraphidium minutum | |
| 3-phosphoglycerate | 2 mM, 86% inhibition; inhibition kinetics | Monoraphidium minutum | |
| ATP | above 0.2 mM, inhibition is alleviated by Mg2+; free form | Monoraphidium minutum | |
| citrate | 2 mM, complete inhibition | Monoraphidium minutum | |
| malate | 2 mM, 92% inhibition | Monoraphidium minutum | |
| additional information | not inhibited by 2-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by glutamine, glutamate | Monoraphidium minutum | |
| phosphoenolpyruvate | 2 mM, complete inhibition; inhibition kinetic | Monoraphidium minutum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Monoraphidium minutum | |
| 0.009 | - |
ATP | pH 7.25, 25°C | Monoraphidium minutum | |
| 0.01 | - |
ATP | - |
Monoraphidium minutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Monoraphidium minutum | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Km: 0.5 mM | Monoraphidium minutum | |
| Mg2+ | required for activity | Monoraphidium minutum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
4 * 70000, SDS-PAGE, smallest catalytically active form | Monoraphidium minutum |
| 280000 | - |
gel filtration, smallest catalytically active form, tends to aggregate to different active forms | Monoraphidium minutum |
| 6000000 | - |
gel filtration, largest catalytically active form, tends to aggregate to different active forms | Monoraphidium minutum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monoraphidium minutum | - |
green alga | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| no isozymes | Monoraphidium minutum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122 | 180 | - |
Monoraphidium minutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Monoraphidium minutum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 70000, SDS-PAGE, smallest catalytically active form | Monoraphidium minutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Monoraphidium minutum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | approx. half-maximal activity at pH 6.5 and pH 8.2 | Monoraphidium minutum |
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