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Literature summary for 2.7.1.11 extracted from

  • Kombrink, E.; Wober, G.
    Chloroplast phosphofructokinase in the green alga, Dunaliella marina: partial purification and kinetic and regulatory properties (1982), Arch. Biochem. Biophys., 213, 602-619.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate kinetics Dunaliella marina
phosphate the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio Dunaliella marina

General Stability

General Stability Organism
very unstable upon purification, dithioerythritol, K+ or Mg2+ and phosphate partially stabilize Dunaliella marina

Inhibitors

Inhibitors Comment Organism Structure
2-phosphoglycerate 1 mM, complete inhibition, phosphate relieves, kinetics Dunaliella marina
3-phosphoglycerate 1 mM, complete inhibition, phosphate relieves, 0.5 mM, 50% inhibition, sigmoidal inhibition curve; inhibition kinetics Dunaliella marina
ADP
-
Dunaliella marina
ATP strong inhibition above 1 mM, at 2.5 mM Mg2+ Dunaliella marina
citrate
-
Dunaliella marina
D-glucose 6-phosphate weak inhibition Dunaliella marina
additional information not inhibited by ADPglucose, dithiothreitol, gluconate 6-phosphate, glucose 1-phosphate; not inhibited by NH4+ and K+ Dunaliella marina
phosphate above 5 mM; kinetics; the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio Dunaliella marina
phosphoenolpyruvate 1 mM, complete inhibition, 0.08 mM, 50% inhibition; inhibition kinetic; phosphate relieves Dunaliella marina
Phosphoglycolate 1 mM, complete inhibition, phosphate relieves, kinetics Dunaliella marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Dunaliella marina
0.06
-
ATP pH 7.0 Dunaliella marina

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast exclusively Dunaliella marina 9507
-
plastid chloroplasts Dunaliella marina 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Dunaliella marina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
282000
-
30 h sucrose density gradient centrifugation Dunaliella marina
434000
-
15 h sucrose density gradient centrifugation Dunaliella marina
644000
-
5 h sucrose density gradient centrifugation Dunaliella marina

Organism

Organism UniProt Comment Textmining
Dunaliella marina
-
green alga
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, partial purification Dunaliella marina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
-
Dunaliella marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Dunaliella marina ADP + D-fructose 1,6-bisphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Dunaliella marina

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 approx. half-maximal activity at pH 6 and pH 8.5 Dunaliella marina