Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | kinetics | Dunaliella marina | |
phosphate | the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio | Dunaliella marina |
General Stability | Organism |
---|---|
very unstable upon purification, dithioerythritol, K+ or Mg2+ and phosphate partially stabilize | Dunaliella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phosphoglycerate | 1 mM, complete inhibition, phosphate relieves, kinetics | Dunaliella marina | |
3-phosphoglycerate | 1 mM, complete inhibition, phosphate relieves, 0.5 mM, 50% inhibition, sigmoidal inhibition curve; inhibition kinetics | Dunaliella marina | |
ADP | - |
Dunaliella marina | |
ATP | strong inhibition above 1 mM, at 2.5 mM Mg2+ | Dunaliella marina | |
citrate | - |
Dunaliella marina | |
D-glucose 6-phosphate | weak inhibition | Dunaliella marina | |
additional information | not inhibited by ADPglucose, dithiothreitol, gluconate 6-phosphate, glucose 1-phosphate; not inhibited by NH4+ and K+ | Dunaliella marina | |
phosphate | above 5 mM; kinetics; the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio | Dunaliella marina | |
phosphoenolpyruvate | 1 mM, complete inhibition, 0.08 mM, 50% inhibition; inhibition kinetic; phosphate relieves | Dunaliella marina | |
Phosphoglycolate | 1 mM, complete inhibition, phosphate relieves, kinetics | Dunaliella marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Dunaliella marina | |
0.06 | - |
ATP | pH 7.0 | Dunaliella marina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | exclusively | Dunaliella marina | 9507 | - |
plastid | chloroplasts | Dunaliella marina | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Dunaliella marina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
282000 | - |
30 h sucrose density gradient centrifugation | Dunaliella marina |
434000 | - |
15 h sucrose density gradient centrifugation | Dunaliella marina |
644000 | - |
5 h sucrose density gradient centrifugation | Dunaliella marina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella marina | - |
green alga | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, partial purification | Dunaliella marina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.38 | - |
- |
Dunaliella marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Dunaliella marina | ADP + D-fructose 1,6-bisphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Dunaliella marina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | approx. half-maximal activity at pH 6 and pH 8.5 | Dunaliella marina |