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Literature summary for 2.7.1.1 extracted from

  • Lazar, Z.; Dulermo, T.; Neuveglise, C.; Crutz-Le Coq, A.M.; Nicaud, J.M.
    Hexokinase - A limiting factor in lipid production from fructose in Yarrowia lipolytica (2014), Metab. Eng., 26, 89-99.
    View publication on PubMed

Application

Application Comment Organism
synthesis overexpression of HXK1 in a highly modified strain of Yarrowia lipolytica W29 engineered to optimize oil production, combined with Saccharomyces cerevisiae invertase gene expression. For the recombinant strain, sucrose is a better substrate than either of its building blocks, glucose or fructose. Over its 96 h of growth in the bioreactors, this strain produces 9.15g/l of lipids, yielding 0.262g/g of biomass Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica O74996
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Synonyms

Synonyms Comment Organism
Hxk1
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Yarrowia lipolytica

General Information

General Information Comment Organism
physiological function Overexpression of the HXK1 gene leads to increased hexokinase activity (6.5-12 times higher), a pronounced reduction in cell filamentation in overexpressing strains grown in fructose-based media, and improved biomass production. All overexpressing strains show improved lipid yield and production when grown on fructose, although th eeffect is strain-dependent (23-55% improvement) Yarrowia lipolytica