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Literature summary for 2.7.1.1 extracted from

  • Nakamura, T.; Kashima, Y.; Mine, S.; Oku, T.; Uegaki, K.
    Characterization and crystal structure of the thermophilic ROK hexokinase from Thermus thermophilus (2012), J. Biosci. Bioeng., 114, 150-154.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme is determined at a resolution of 2.02 A, with Rcryst and Rfree values of 18.1% and 22.6%, respectively Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
D-glucose pH 7.0, 50°C Thermus thermophilus
0.37
-
D-mannose pH 7.0, 50°C Thermus thermophilus
105
-
D-galactose pH 7.0, 50°C Thermus thermophilus
1510
-
D-fructose pH 7.0, 50°C Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SLJ4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
pH 7.0, 50°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Thermus thermophilus ADP + D-fructose 6-phosphate
-
?
ATP + D-galactose
-
Thermus thermophilus ADP + D-galactose 6-phosphate
-
?
ATP + D-glucose
-
Thermus thermophilus ADP + D-glucose 6-phosphate
-
?
ATP + D-mannose
-
Thermus thermophilus ADP + D-mannose 6-phosphate
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Thermus thermophilus

Synonyms

Synonyms Comment Organism
ROK hexokinase
-
Thermus thermophilus
TthHK
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
TthHK is stable at 90°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Thermus thermophilus