Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phosphoglycerol | - |
Lithobates sylvaticus | |
Urea | - |
Lithobates sylvaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.139 | - |
D-glucose | control, pH 8.2, 5°C, no glucose | Lithobates sylvaticus | |
0.144 | - |
D-glucose | control, pH 8.2, 25°C | Lithobates sylvaticus | |
0.144 | - |
D-glucose | control, pH 8.2, 25°C, no glucose | Lithobates sylvaticus | |
0.239 | - |
ATP | control, pH 8.2, 25°C, 250 mM glucose | Lithobates sylvaticus | |
0.248 | - |
ATP | control, pH 8.2, 25°C | Lithobates sylvaticus | |
0.248 | - |
ATP | control, pH 8.2, 25°C, no glucose | Lithobates sylvaticus | |
0.248 | - |
D-glucose | frozen, pH 8.2, 25°C | Lithobates sylvaticus | |
0.248 | - |
D-glucose | frozen, pH 8.2, 25°C, no glucose | Lithobates sylvaticus | |
0.253 | - |
D-glucose | frozen, pH 8.2, 5°C, no glucose | Lithobates sylvaticus | |
0.257 | - |
ATP | control, pH 8.2, 5°C, 250 mM glucose | Lithobates sylvaticus | |
0.33 | - |
ATP | frozen, pH 8.2, 25°C | Lithobates sylvaticus | |
0.33 | - |
ATP | frozen, pH 8.2, 25°C, no glucose | Lithobates sylvaticus | |
0.336 | - |
ATP | frozen, pH 8.2, 5°C, 250 mM glucose | Lithobates sylvaticus | |
0.342 | - |
ATP | frozen, pH 8.2, 25°C, 250 mM glucose | Lithobates sylvaticus | |
0.364 | - |
ATP | control, pH 8.2, 5°C, no glucose | Lithobates sylvaticus | |
0.505 | - |
ATP | frozen, pH 8.2, 5°C, no glucose | Lithobates sylvaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Lithobates sylvaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithobates sylvaticus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | differential phosphorylation of hexokinase is responsible for altered kinetic properties: in control frogs, hexokinase is in a higher phosphate form and displays a high substrate affinity and high activity, whereas in frozen frogs hexokinase is less phosphorylated, with lower substrate affinity and lower activity | Lithobates sylvaticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Lithobates sylvaticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Lithobates sylvaticus | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hexokinase | - |
Lithobates sylvaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lithobates sylvaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
optimal pH | Lithobates sylvaticus |
8.2 | - |
assay at | Lithobates sylvaticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.4 | 90% activity, frozen frogs | Lithobates sylvaticus |
8.1 | 8.6 | 90% activity, control frogs | Lithobates sylvaticus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0045 | - |
frozen, pH 8.2, 25°C | Lithobates sylvaticus | Urea | |
0.0053 | - |
control, pH 8.2, 25°C | Lithobates sylvaticus | Urea |