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Literature summary for 2.7.1.1 extracted from

  • Dieni, C.A.; Storey, K.B.
    Regulation of hexokinase by reversible phosphorylation in skeletal muscle of a freeze-tolerant frog (2011), Comp. Biochem. Physiol. B, 159, 236-243.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-Phosphoglycerol
-
Lithobates sylvaticus
Urea
-
Lithobates sylvaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
D-glucose control, pH 8.2, 5°C, no glucose Lithobates sylvaticus
0.144
-
D-glucose control, pH 8.2, 25°C Lithobates sylvaticus
0.144
-
D-glucose control, pH 8.2, 25°C, no glucose Lithobates sylvaticus
0.239
-
ATP control, pH 8.2, 25°C, 250 mM glucose Lithobates sylvaticus
0.248
-
ATP control, pH 8.2, 25°C Lithobates sylvaticus
0.248
-
ATP control, pH 8.2, 25°C, no glucose Lithobates sylvaticus
0.248
-
D-glucose frozen, pH 8.2, 25°C Lithobates sylvaticus
0.248
-
D-glucose frozen, pH 8.2, 25°C, no glucose Lithobates sylvaticus
0.253
-
D-glucose frozen, pH 8.2, 5°C, no glucose Lithobates sylvaticus
0.257
-
ATP control, pH 8.2, 5°C, 250 mM glucose Lithobates sylvaticus
0.33
-
ATP frozen, pH 8.2, 25°C Lithobates sylvaticus
0.33
-
ATP frozen, pH 8.2, 25°C, no glucose Lithobates sylvaticus
0.336
-
ATP frozen, pH 8.2, 5°C, 250 mM glucose Lithobates sylvaticus
0.342
-
ATP frozen, pH 8.2, 25°C, 250 mM glucose Lithobates sylvaticus
0.364
-
ATP control, pH 8.2, 5°C, no glucose Lithobates sylvaticus
0.505
-
ATP frozen, pH 8.2, 5°C, no glucose Lithobates sylvaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE Lithobates sylvaticus

Organism

Organism UniProt Comment Textmining
Lithobates sylvaticus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein differential phosphorylation of hexokinase is responsible for altered kinetic properties: in control frogs, hexokinase is in a higher phosphate form and displays a high substrate affinity and high activity, whereas in frozen frogs hexokinase is less phosphorylated, with lower substrate affinity and lower activity Lithobates sylvaticus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Lithobates sylvaticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
Lithobates sylvaticus ADP + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
hexokinase
-
Lithobates sylvaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Lithobates sylvaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
optimal pH Lithobates sylvaticus
8.2
-
assay at Lithobates sylvaticus

pH Range

pH Minimum pH Maximum Comment Organism
7.9 8.4 90% activity, frozen frogs Lithobates sylvaticus
8.1 8.6 90% activity, control frogs Lithobates sylvaticus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0045
-
frozen, pH 8.2, 25°C Lithobates sylvaticus Urea
0.0053
-
control, pH 8.2, 25°C Lithobates sylvaticus Urea