Any feedback?
Literature summary for 2.7.1.1 extracted from
- 23-Residue C-terminal alpha-helix governs kinetic cooperativity in monomeric human glucokinase (2009), Biochemistry, 48, 6157-6165.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild type and mutant enzymes are expressed as N-terminal hexa-histidine tagged polypeptides in glucokinase-deficient Escherichia coli K-12 strain BM5340(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D205A | mutant shows almost no activity | Homo sapiens |
| additional information | removal of helix alpha13 abolishes cooperativity and restores Michaelis-Menten kinetics, while reducing the kcat value of the wild type enzyme by 160fold, the impaired catalytic activity of the truncated enzyme is not rescued by the trans-addition of a synthetic alpha13 peptide | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superose 6 gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucokinase | - |
Homo sapiens |
| hexokinase IV | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00091 | - |
D-glucose | mutant enzyme D205A, kcat less than 0.00091 s-1 | Homo sapiens | |
| 0.18 | - |
D-glucose | truncated enzyme with removed helix alpha13 | Homo sapiens | |
| 29 | - |
D-glucose | wild type enzyme | Homo sapiens | |
| 37 | - |
D-glucose | glucokinase with C-terminal 5 alanine addition | Homo sapiens | |
| 38 | - |
D-glucose | glucokinase with C-terminal 10 alanine addition | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
