Literature summary for 2.7.1.1 extracted from

Jeong, E.J.; Park, K.; Yi, S.Y.; Kang, H.J.; Chung, S.J.; Lee, C.S.; Chung, J.W.; Seol, D.W.; Chung, B.H.; Kim, M.
Stress-governed expression and purification of human type II hexokinase in Escherichia coli (2007), J. Microbiol. Biotechnol., 17, 638-643.

Cloned(Commentary)

Cloned (Comment)	Organism
the full encoding sequence for human type II hexokinase is cloned into the Escherichia coli expression vector pET 21b and expressed as a C-terminally hexahistidine-tagged protein in the BL21 strain. The IPTG-induced HXK II approximately accounts for 17% of the total Escherichia coli proteins, and 81% of hexahistidine-tagged HXK II exists in inclusion bodies. The production of soluble recombinant HXK II protein, in the functionally active form, is improved by low temperature, and the osmotic stress expression method. When expressed at 18°C, about 83% of HXK II-6His exists in the soluble fraction, which amounts to a 4.1fold yield over that expressed at 37°C. The soluble form of hexahistidine-tagged enzyme is also highly produced in the presence of 1 M sorbitol under the standard condition (37°C), which indicates that temperature downshift and low water potentials are required to improve the yield of active recombinant HXK II protein	Homo sapiens

Cloned (Comment)

Organism

the full encoding sequence for human type II hexokinase is cloned into the Escherichia coli expression vector pET 21b and expressed as a C-terminally hexahistidine-tagged protein in the BL21 strain. The IPTG-induced HXK II approximately accounts for 17% of the total Escherichia coli proteins, and 81% of hexahistidine-tagged HXK II exists in inclusion bodies. The production of soluble recombinant HXK II protein, in the functionally active form, is improved by low temperature, and the osmotic stress expression method. When expressed at 18°C, about 83% of HXK II-6His exists in the soluble fraction, which amounts to a 4.1fold yield over that expressed at 37°C. The soluble form of hexahistidine-tagged enzyme is also highly produced in the presence of 1 M sorbitol under the standard condition (37°C), which indicates that temperature downshift and low water potentials are required to improve the yield of active recombinant HXK II protein

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P52789	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Homo sapiens

Synonyms

Synonyms	Comment	Organism
HXK II	-	Homo sapiens
type II hexokinase	-	Homo sapiens

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Release 2023.1 (January 2023)