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Literature summary for 2.7.1.1 extracted from
- Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii (2006), J. Bacteriol., 188, 2014-2019.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfurisphaera tokodaii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 1 mM, 92% inhibition. Competitive inhibition versus ATP, noncompetitive inhibition versus D-glucose | Sulfurisphaera tokodaii |  |
| AMP | 1 mM, 73% inhibition | Sulfurisphaera tokodaii | |
| CDP | 1 mM, 33% inhibition | Sulfurisphaera tokodaii | |
| D-fructose 6-phosphate | 10 mM, 33% inhibition | Sulfurisphaera tokodaii | |
| diphosphate | 10 mM, 54% inhibition | Sulfurisphaera tokodaii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
D-glucose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
| 0.12 | - |
ATP | pH 7.5, 50°C, D-glucose as phosphoryl acceptor | Sulfurisphaera tokodaii | |
| 0.13 | - |
D-mannose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
| 0.23 | - |
D-glucosamine | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
| 0.32 | - |
N-acetyl-D-glucosamine | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
| 0.44 | - |
2-deoxy-D-glucose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
| 0.52 | - |
CTP | pH 7.5, 50°C, D-glucose as phosphoryl acceptor | Sulfurisphaera tokodaii | |
| 30 | - |
D-fructose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
| Mg2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
| Mn2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
| additional information | no activity in the presence of Zn2+ | Sulfurisphaera tokodaii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Sulfurisphaera tokodaii |
| 32100 | - |
2 * 32100, calculated from sequence | Sulfurisphaera tokodaii |
| 64000 | - |
gel filtration | Sulfurisphaera tokodaii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Sulfurisphaera tokodaii | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | Sulfurisphaera tokodaii 7 | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | Q96Y14 | - |
- |
| Sulfurisphaera tokodaii 7 | Q96Y14 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
pH 7.5, 50°C, substrate: D-glucose | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | - |
Sulfurisphaera tokodaii | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + 2-deoxy-D-glucose | - |
Sulfurisphaera tokodaii 7 | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + D-fructose | - |
Sulfurisphaera tokodaii | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucosamine | - |
Sulfurisphaera tokodaii | ADP + D-glucosamine 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Sulfurisphaera tokodaii | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | Sulfurisphaera tokodaii | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Sulfurisphaera tokodaii 7 | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | Sulfurisphaera tokodaii 7 | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Sulfurisphaera tokodaii | ADP + D-mannose 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | - |
Sulfurisphaera tokodaii | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| CTP + D-glucose | 81% of the activity with ATP | Sulfurisphaera tokodaii | CDP + D-glucose 6-phosphate | - |
? | |
| GTP + D-glucose | 52% of the activity with ATP | Sulfurisphaera tokodaii | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | 64% of the activity with ATP | Sulfurisphaera tokodaii | IDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | 64% of the activity with ATP | Sulfurisphaera tokodaii 7 | IDP + D-glucose 6-phosphate | - |
? | |
| additional information | galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme | Sulfurisphaera tokodaii 7 | ? | - |
? | |
| UTP + D-glucose | 37% of the activity with ATP | Sulfurisphaera tokodaii | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | Sulfurisphaera tokodaii |
| dimer | 2 * 32100, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-dependent hexokinase | - |
Sulfurisphaera tokodaii |
| STK_23540 | locus name | Sulfurisphaera tokodaii |
| StoHK | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
pH 7.5, 120 min, 14% loss of activity | Sulfurisphaera tokodaii |
| 90 | - |
pH 7.5, 120 min, 80% loss of activity | Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | more than 60% of the maximal activity in the pH range of 6.5 to 9.0 | Sulfurisphaera tokodaii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
ADP | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
D-fructose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 52 | - |
CTP | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 100 | - |
N-acetyl-D-glucosamine | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 170 | - |
2-deoxy-D-glucose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 247 | - |
D-glucosamine | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 259 | - |
ATP | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 420 | - |
D-mannose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
| 717 | - |
D-glucose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
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