Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, recombinant TbHK2 lacks detectable enzymatic activity. Replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1 | Trypanosoma brucei |
expression in Escherichia coli, TbHK1 | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | replacing the last 18 amino acids of TbHK1 with the corresponding residues of TbHK2 yields an inactive recombinant protein | Trypanosoma brucei |
additional information | replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1 | Trypanosoma brucei |
N469D | inactive mutant protein, TbHK1 | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lauric acid | IC50: 0.0758 mM, TbHK1 | Trypanosoma brucei | |
myristic acid | IC50: 0.0784 mM, TbHK1 | Trypanosoma brucei | |
palmitic acid | IC50: 0.0624 mM, TbHK1 | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
D-glucose | recombinant TbHK1 | Trypanosoma brucei | |
0.03 | - |
D-mannose | recombinant TbHK1 | Trypanosoma brucei | |
0.035 | - |
D-fructose | recombinant TbHK1 | Trypanosoma brucei | |
0.28 | - |
ATP | recombinant TbHK1 | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q95PL2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | - |
Trypanosoma brucei | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Trypanosoma brucei | ADP + D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | - |
Trypanosoma brucei | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | - |
Trypanosoma brucei | ADP + D-glucosamine 6-phosphate | - |
? | |
D-mannose + ATP | - |
Trypanosoma brucei | ADP + D-mannose 6-phosphate | - |
? | |
additional information | the C-terminal tail of TbHK1 is important for hexokinase activity | Trypanosoma brucei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TbHK1 | - |
Trypanosoma brucei |
TbHK2 | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
483.3 | - |
D-glucose | recombinant TbHK1 | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0624 | - |
IC50: 0.0624 mM, TbHK1 | Trypanosoma brucei | palmitic acid | |
0.0758 | - |
IC50: 0.0758 mM, TbHK1 | Trypanosoma brucei | lauric acid | |
0.0784 | - |
IC50: 0.0784 mM, TbHK1 | Trypanosoma brucei | myristic acid |