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Literature summary for 2.7.1.1 extracted from

  • Olsen, S.N.; Ramlov, H.; Westh, P.
    Effects of osmolytes on hexokinase kinetics combined with macromolecular crowding Test of the osmolyte compatibility hypothesis towards crowded systems (2007), Comp. Biochem. Physiol. A, 148, 339-345.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information osmolytes decrease kcat/KM in the order of decreasing efficiency NaCl, urea, trimethylamine N-oxide/glycerol, betaine. For the organic osmolytes this order correlates with the degree of exclusion from protein/water interfaces Saccharomyces cerevisiae
additional information
-
additional information the osmolytes decreases kcat/KM in the order: NaCl/urea/trimethylamine-N-oxide dehydrate/glycerol/betaine. For the organic osmolytes this order correlates with the degree of exclusion from protein-water interfaces. Thus, the stronger the exclusion the weaker the perturbing effects on kcat/KM Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information osmolytes decrease kcat/KM in the order ofedecreasing efficiency NaCl, urea, trimethylamine N-oxide/glycerol, betains. For the organic osmolytes this order correlates with the degree of exclusion from protein/water interfaces Saccharomyces cerevisiae
additional information
-
additional information the osmolytes decreases kcat/KM in the order: NaCl/urea/trimethylamine-N-oxide dehydrate/glycerol/betaine. For the organic osmolytes this order correlates with the degree of exclusion from protein-water interfaces. Thus, the stronger the exclusion the weaker the perturbing effects on kcat/KM Saccharomyces cerevisiae