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Literature summary for 2.7.1.1 extracted from
- Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41 (2007), Appl. Environ. Microbiol., 73, 3581-3586.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lysinibacillus sphaericus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
D-glucose | - |
Lysinibacillus sphaericus |  |
| 0.52 | - |
ATP | - |
Lysinibacillus sphaericus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | A0SZU9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lysinibacillus sphaericus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | glucokinase is continuously expressed during the all growth stages, and the peak value for glucokinase activity occurrs in the stationary growth phase before spores form | Lysinibacillus sphaericus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.786 | - |
substrate: D-fructose | Lysinibacillus sphaericus |
| 0.941 | - |
substrate: D-mannose | Lysinibacillus sphaericus |
| 1.95 | - |
substrate: D-glucose | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Lysinibacillus sphaericus | ? | - |
? | |
| ATP + D-glucose | - |
Lysinibacillus sphaericus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Lysinibacillus sphaericus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-phosphate glucose kinase | - |
Lysinibacillus sphaericus |
| GlcK | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Lysinibacillus sphaericus |
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Release 2023.1 (January 2023)
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