Literature summary for 2.7.1.1 extracted from

Romero, C.S.; Olmo, R.; Teijon, C.; Blanco, M.D.; Teijon, J.M.; Romero, A.
Structural and functional implications of the hexokinase-nickel interaction (2005), J. Inorg. Biochem., 99, 2395-2402.

Search for more literature for 2.7.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
Ni2+	competitive versus ATP via replacement of Mg2+, noncompetitive versus D-glucose via a cysteine residue proximal to the D-glucose binding site, enzyme-nickel interactions with positive cooperativity via histidine residues, no saturation is reached, nickel binding induces conformational changes in the secondary structure of the enzyme modifying the monomer/dimer equilibrium and decreasing the activity, overview	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-glucose	Saccharomyces cerevisiae	-	ADP + D-glucose 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + D-hexose = ADP + D-hexose 6-phosphate	catalytic residues in the D-glucose binding site are Glu269, Glu302, and Asp211	Saccharomyces cerevisiae	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Saccharomyces cerevisiae	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-glucose	-	Saccharomyces cerevisiae	ADP + D-glucose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	at lower Ni2+ concentration	Saccharomyces cerevisiae
monomer	at very high Ni2+ concentration	Saccharomyces cerevisiae
More	enzyme-nickel interactions modify the monomer/dimer equilibrium, overview	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.87	-	Ni2+	pH 7.6, 37°C, versus ATP	Saccharomyces cerevisiae
9.97	-	Ni2+	pH 7.6, 37°C, versus D-glucose	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)