Cloned (Comment) | Organism |
---|---|
complementary cloning in the glucose-growth-deficient double kinase-negative mutant strain A31-10, DNA and amino acid sequence determination and analysis, phylogenetic tree | Ogataea angusta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Ogataea angusta | |
trehalose 6-phosphate | competitive to D-glucose | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
D-glucose | pH 7.5, 37°C | Ogataea angusta | |
1.1 | - |
D-fructose | pH 7.5, 37°C | Ogataea angusta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54200 | - |
x * 54200, about, sequence calculation | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Ogataea angusta | enzyme mediates glucose repression | ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | Q96VP5 | methylotrophic yeast | - |
Purification (Comment) | Organism |
---|---|
106fold by 2 steps of ion exchange chromatography, and gel filtration | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in different strains, overview | Ogataea angusta |
95.7 | - |
purified enzyme, substrate D-glucose | Ogataea angusta |
268.8 | - |
purified enzyme, substrate D-fructose | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | 17% of activity with D-fructose at 50 mM substrate concentration | Ogataea angusta | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-fructose | best substrate | Ogataea angusta | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucosamine | 8% of activity with D-fructose at 50 mM substrate concentration | Ogataea angusta | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucose | enzyme mediates glucose repression | Ogataea angusta | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | 39% of activity with D-fructose at 50 mM substrate concentration | Ogataea angusta | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | 33% of activity with D-fructose at 50 mM substrate concentration | Ogataea angusta | ADP + D-mannose 6-phosphate | - |
? | |
additional information | no activity with D-xylose, D-galactose, and methyl-alpha-D-glucoside | Ogataea angusta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54200, about, sequence calculation | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
HPGLK1 | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Ogataea angusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | 91% of maximal activity at pH 7.5, maximal activity at pH 8.5 | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ogataea angusta |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
trehalose 6-phosphate | pH 7.5, 37°C | Ogataea angusta |