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Literature summary for 2.7.1.1 extracted from

  • Karp, H.; Jarviste, A.; Kriegel, T.M.; Alamae, T.
    Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha (2003), Curr. Genet., 44, 268-276.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
complementary cloning in the glucose-growth-deficient double kinase-negative mutant strain A31-10, DNA and amino acid sequence determination and analysis, phylogenetic tree Ogataea angusta

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Ogataea angusta
trehalose 6-phosphate competitive to D-glucose Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 D-glucose pH 7.5, 37°C Ogataea angusta
1.1
-
 D-fructose pH 7.5, 37°C Ogataea angusta

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54200
-
 x * 54200, about, sequence calculation Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Ogataea angusta enzyme mediates glucose repression ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea angusta Q96VP5 methylotrophic yeast
-

Purification (Commentary)

Purification (Comment) Organism
106fold by 2 steps of ion exchange chromatography, and gel filtration Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in different strains, overview Ogataea angusta
95.7
-
 purified enzyme, substrate D-glucose Ogataea angusta
268.8
-
 purified enzyme, substrate D-fructose Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose 17% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
ATP + D-fructose best substrate Ogataea angusta ADP + D-fructose 6-phosphate
-
 ?
ATP + D-glucosamine 8% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-glucosamine 6-phosphate
-
 ?
ATP + D-glucose enzyme mediates glucose repression Ogataea angusta ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose 39% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose 33% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-mannose 6-phosphate
-
 ?
additional information no activity with D-xylose, D-galactose, and methyl-alpha-D-glucoside Ogataea angusta ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54200, about, sequence calculation Ogataea angusta

Synonyms

Synonyms Comment Organism
HPGLK1
-
 Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Ogataea angusta

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 91% of maximal activity at pH 7.5, maximal activity at pH 8.5 Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Ogataea angusta

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
 trehalose 6-phosphate pH 7.5, 37°C Ogataea angusta