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Literature summary for 2.7.1.1 extracted from

  • Sebastian, S.; Wilson, J.E.; Mulichak, A.; Garavito, R.M.
    Allosteric regulation of type I hexokinase: A site-directed mutational study indicating location of the functional glucose 6-phosphate binding site in the N-terminal half of the enzyme (1999), Arch. Biochem. Biophys., 362, 203-210.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding HK I is cloned and expressed in M+R42 cells Rattus norvegicus
mutant HK I is cloned and expressed in M+R42 cells Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
hexokinase I from brain complexed with glucose and phosphate Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D532E HK I mutant Rattus norvegicus
D532K HK I mutant Rattus norvegicus
D532N HK I mutant Rattus norvegicus
D84A HK I mutant with 2fold increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, mutation diminishes the ability of phosphate to antagonize inhibition, but has no effect on the inhibition by phosphate at higher concentrations Rattus norvegicus
D84E HK I mutant, slightly increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, antagonistic effect of phosphate is drastically reduced, no effect on the inhibition by phosphate at higher concentrations Rattus norvegicus
D84K HK I mutant, increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, antagonistic effect of phosphate is abolished, slightly decreased Ki value for inhibition by phosphate at higher concentrations Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,5-Anhydro-D-glucitol 6-phosphate hexokinase I, antagonism by phosphate at low concentrations Rattus norvegicus
D-glucose 6-phosphate product inhibition of hexokinase I, antagonism by phosphate at low concentrations results from competition for a common anion binding site located in the N-terminal half Rattus norvegicus
phosphate competitive versus ATP; hexokinase I, at high concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035 0.038 D-glucose recombinant wild-type hexokinase I, D84A, D84E and D84K mutants Rattus norvegicus
0.43 0.47 ATP recombinant wild-type hexokinase I, D84A, D84E and D84K mutants Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P05708 recombinant hexokinase type I
-

Purification (Commentary)

Purification (Comment) Organism
hexokinase I from brain Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain hexokinase I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP
-
 Rattus norvegicus ADP + D-glucose 6-phosphate
-
 ?
additional information structure model of hexokinase I Rattus norvegicus ?
-
 ?
additional information allosteric regulation of hexokinase I, the functional regulatory glucose 6-phosphate binding site is located in the N-terminal half Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer in the crystal structure Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 1,5-Anhydro-D-glucitol 6-phosphate recombinant wild-type hexokinase I Rattus norvegicus
0.018
-
 1,5-Anhydro-D-glucitol 6-phosphate recombinant D84E mutant hexokinase I Rattus norvegicus
0.028
-
 1,5-Anhydro-D-glucitol 6-phosphate recombinant D84K mutant hexokinase I Rattus norvegicus
0.033
-
 1,5-Anhydro-D-glucitol 6-phosphate recombinant D84A mutant hexokinase I Rattus norvegicus
13
-
 phosphate recombinant D84K mutant hexokinase I Rattus norvegicus
15
-
 phosphate recombinant D84E mutant hexokinase I Rattus norvegicus
17
-
 phosphate recombinant wild-type hexokinase I Rattus norvegicus
18
-
 phosphate recombinant D84A mutant hexokinase I Rattus norvegicus