Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Aspergillus niger | |
citrate | noncompetitive to glucose and ATP | Aspergillus niger | |
Mg2+ | enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
Mn2+ | enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
additional information | not inhibited by D-glucose 6-phosphate | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
D-glucose | - |
Aspergillus niger | |
0.54 | - |
ATP | - |
Aspergillus niger | |
29 | - |
D-fructose | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
Mn2+ | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Aspergillus niger |
100000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
strain ATCC 11414 | - |
Purification (Comment) | Organism |
---|---|
about 600fold | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | glucose-grown | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
D-fructose | Aspergillus niger |
40 | - |
D-glucose | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | - |
Aspergillus niger | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | - |
Aspergillus niger | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | - |
Aspergillus niger | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | - |
Aspergillus niger | ADP + D-mannose 6-phosphate | - |
? | |
additional information | not: D-galactose | Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
high activity over a broad pH-range | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
citrate | - |
Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | analytical chromatofocusing, at 23°C | 5.9 | 5.6 |