Any feedback?
Literature summary for 2.7.1.1 extracted from
- Characterization and regulatory properties of a single hexokinase from the citric acid accumulating fungus Aspergillus niger (1994), Biochim. Biophys. Acta, 1200, 215-223.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Aspergillus niger |  |
| citrate | noncompetitive to glucose and ATP | Aspergillus niger | |
| Mg2+ | enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
| Mn2+ | enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
| additional information | not inhibited by D-glucose 6-phosphate | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
D-glucose | - |
Aspergillus niger | |
| 0.54 | - |
ATP | - |
Aspergillus niger | |
| 29 | - |
D-fructose | - |
Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
| Mn2+ | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Aspergillus niger |
| 100000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
strain ATCC 11414 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 600fold | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | glucose-grown | Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | - |
D-fructose | Aspergillus niger |
| 40 | - |
D-glucose | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | - |
Aspergillus niger | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| D-fructose + ATP | - |
Aspergillus niger | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucose + ATP | - |
Aspergillus niger | ADP + D-glucose 6-phosphate | - |
? | |
| D-mannose + ATP | - |
Aspergillus niger | ADP + D-mannose 6-phosphate | - |
? | |
| additional information | not: D-galactose | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
high activity over a broad pH-range | Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ | Aspergillus niger | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
citrate | - |
Aspergillus niger | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | analytical chromatofocusing, at 23°C | 5.9 | 5.6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
