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Literature summary for 2.7.1.1 extracted from

  • Renz, A.; Merlo, L.; Stitt, M.
    Partial purification from potato tubers of three fructokinases and three hexokinases which show differing organ and developmental specificity (1993), Planta, 190, 156-165.
No PubMed abstract available
Search for more literature for 2.7.1.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 HK1 and HK2, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Solanum tuberosum organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 cv. Desiree, 3 isoenzymes: HK1, HK2, HK3
-

Purification (Commentary)

Purification (Comment) Organism
3 isoenzymes: HK1: 140fold, HK2: 40fold, HK3: 25fold Solanum tuberosum
partial Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber developing Solanum tuberosum
-
tuber organ- and development-specific changes in the abundance of the 3 isoenzymes Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 HK3, at pH 8 Solanum tuberosum
0.72
-
 HK2, at pH 8 Solanum tuberosum
0.84
-
 HK1, at pH 8 Solanum tuberosum

Storage Stability

Storage Stability Organism
-180°C, 2 months, stable Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + ATP HK3: 16% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
D-fructose + ATP HK1: 4% of the activity with glucose, HK2: 3% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
D-glucose + ATP
-
 Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Solanum tuberosum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Solanum tuberosum