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Literature summary for 2.7.1.1 extracted from

  • Stocchi, V.; Magnani, M.; Canestrari, F.; Dacha, M.; Fornaini, G.
    Rabbit red blood cell hexokinase. Evidence for two distinct forms, and their purification and characterization from reticulocytes (1981), J. Biol. Chem., 256, 7856-7862.
    View publication on PubMed
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
D-glucose or D-fructose, 5 mM, stabilizes Oryctolagus cuniculus
glycerol, 9-15% v/v, stabilizes Oryctolagus cuniculus
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 D-glucose reticulocyte hexokinase Ia Oryctolagus cuniculus
0.04
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
0.1
-
 D-mannose hexokinase Ia Oryctolagus cuniculus
0.125
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
0.125
-
 D-glucose reticulocyte hexokinase Ib Oryctolagus cuniculus
0.15
-
 D-mannose hexokinase Ib Oryctolagus cuniculus
0.5
-
 ATP MgATP2- Oryctolagus cuniculus
0.5
-
 ATP reticulocyte hexokinase Ia and Ib, at 37°C, pH 8.1 Oryctolagus cuniculus
0.85
-
 D-glucosamine hexokinase Ia Oryctolagus cuniculus
0.9
-
 2-deoxy-D-glucose hexokinase Ia Oryctolagus cuniculus
1.3
-
 D-glucosamine hexokinase Ib Oryctolagus cuniculus
2
-
 2-deoxy-D-glucose hexokinase Ib Oryctolagus cuniculus
4
-
 D-fructose hexokinase Ia Oryctolagus cuniculus
4.5
-
 ITP MgITP2-, hexokinase Ib Oryctolagus cuniculus
12
-
 ITP MgITP2-, hexokinase Ia Oryctolagus cuniculus
25
-
 D-fructose hexokinase Ib Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble hexokinase Ia and Ib Oryctolagus cuniculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus
105000
-
 reticulocyte hexokinase Ia and Ib, gel filtration, sucrose density gradient sedimentation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 hexokinase Ia and Ib
-

Purification (Commentary)

Purification (Comment) Organism
erythrocyte enzyme and reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
hexokinase Ia: 55460fold, hexokinase Ib: 51260fold Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
144
-
 pH 8.1 Oryctolagus cuniculus
144
-
 reticulocyte, hexokinase Ib, at 37°C Oryctolagus cuniculus
155
-
 pH 8.1 Oryctolagus cuniculus
155
-
 reticulocyte, hexokinase Ia, at 37°C Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-25°C, over a period of months, stable Oryctolagus cuniculus
37°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ib is more stable than hexokinase Ia Oryctolagus cuniculus
4°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ia is more stable than hexokinase Ib Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose hexokinase Ia: 65% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
CTP + D-glucose
-
 Oryctolagus cuniculus CDP + D-glucose 6-phosphate
-
 ?
D-fructose + ATP hexokinase Ia: 69% of the activity with glucose, hexokinase Ib: 42% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate
-
 ?
D-glucosamine + ATP hexokinase Ia: 35% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP MgATP2- Oryctolagus cuniculus ADP + D-glucose 6-phosphate
-
 ?
D-mannose + ATP 104-105% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate
-
 ?
ITP + D-glucose 30% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
additional information not: UTP Oryctolagus cuniculus ?
-
 ?
additional information not: N-acetyl-D-glucosamine Oryctolagus cuniculus ?
-
 ?
additional information not: D-galactose Oryctolagus cuniculus ?
-
 ?
additional information not: GTP Oryctolagus cuniculus ?
-
 ?
additional information not: CTP Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.2
-
 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Oryctolagus cuniculus

pI Value

Organism Comment pI Value Maximum pI Value
Oryctolagus cuniculus reticulocyte hexokinase Ib
-
 5.7
Oryctolagus cuniculus isoelectric focusing
-
 5.7
Oryctolagus cuniculus hexokinase Ia, isoelectric focusing
-
 6.2