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Literature summary for 2.7.1.1 extracted from

  • Liu, F.; Dong, Q.; Myers, A.M.; Fromm, H.J.
    Expression of human brain hexokinase in Escherichia coli: Purification and characterization of the expressed enzyme (1991), Biochem. Biophys. Res. Commun., 177, 305-311.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a synthetic gene, encoding hexokinase I from brain, is expressed in Escherichia coli BL21(DE3) under the control of the bacteriophage T7 promoter Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
human enzyme expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate hexokinase I inhibition is relieved by inorganic phosphate, but not the inhibition of hexokinase II Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
D-glucose recombinant hexokinase I expressed in Escherichia coli Homo sapiens
0.3
-
ATP recombinant hexokinase I expressed in Escherichia coli Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
recombinant hexokinase I expressed in Escherichia coli, deduced from nucleotide sequence of the expressed cDNA and gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Homo sapiens hexokinase I is a regulated enzyme and serves a critical role in controlling the rate of glycolysis in both brain and red blood cells D-glucose 6-phosphate + ADP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant hexokinase I, expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
13fold Homo sapiens
recombinant hexokinase I expressed in Escherichia coli BL21(DE3) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.2
-
recombinant hexokinase I expressed in E. coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP
-
Homo sapiens ADP + D-glucose 6-phosphate
-
?
D-glucose + ATP hexokinase I is a regulated enzyme and serves a critical role in controlling the rate of glycolysis in both brain and red blood cells Homo sapiens D-glucose 6-phosphate + ADP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 100000, recombinant hexokinase I expressed in Escherichia coli, SDS-PAGE Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens