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Literature summary for 2.7.1.1 extracted from

  • Supowit, S.C.; Harris, B.G.
    Hexokinase from Ascaris suum muscle (1982), Methods Enzymol., 90, 21-25.
No PubMed abstract available
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
D-glucose, 0.1 M, stabilizes, purified enzyme is extremely unstable in absence of glucose Ascaris suum

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate
-
 Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 ATP
-
 Ascaris suum
4.7
-
 D-glucose
-
 Ascaris suum
80
-
 D-fructose
-
 Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000 100000 gel filtration Ascaris suum

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
146fold Ascaris suum

Reaction

Reaction Comment Organism Reaction ID
ATP + D-hexose = ADP + D-hexose 6-phosphate random kinetic mechanism Ascaris suum
a

Source Tissue

Source Tissue Comment Organism Textmining
muscle pink-to-red muscle cuticle, fresh, freezing causes substantial loss of hexokinase activity, only one enzyme form Ascaris suum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
 at 30 °C Ascaris suum

Storage Stability

Storage Stability Organism
4°C, 2.76 M ammonium sulfate, 0.1 M glucose, 10 mM 2-mercaptoethanol, per month, 10% loss of activity Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-dATP + D-glucose 73-80% of the activity with ATP, isoenzyme Hex A, B, C Ascaris suum 2'-dADP + D-glucose 6-phosphate
-
 ?
ATP + 2-deoxy-D-glucose
-
 Ascaris suum ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
CTP + D-glucose 11% of the activity with ATP, isoenzyme Hex C, not: isoenzyme Hex A and Hex B Ascaris suum CDP + D-glucose 6-phosphate
-
 ?
D-fructose + ATP
-
 Ascaris suum ADP + D-fructose 6-phosphate
-
 ?
D-glucose + ATP
-
 Ascaris suum ADP + D-glucose 6-phosphate
-
 ?
additional information not: UTP Ascaris suum ?
-
 ?
additional information not: GTP Ascaris suum ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 97000-100000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Ascaris suum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Ascaris suum

pI Value

Organism Comment pI Value Maximum pI Value
Ascaris suum
-
-
 5.9