Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | noncompetitive to glucose, competitive to ATP | Schizosaccharomyces pombe | |
Mg2+ | at 10 mM, slight | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation is associated with an increase in the maximal velocity by its interaction with the ternary complex to facilitate the transfer of the phosphoryl group | Schizosaccharomyces pombe | |
Mg2+ | strict requirement for divalent metal ions | Schizosaccharomyces pombe | |
Mg2+ | maximal activity at 1 mM Mg2+, can be partially replaced by Mn2+ | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
2 * 47500, SDS-PAGE | Schizosaccharomyces pombe |
88000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Schizosaccharomyces pombe | enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway | D-glucose 6-phosphate + ADP | - |
? | |
D-glucose + ATP | Schizosaccharomyces pombe NCYC 132S2-2 | enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
2 isozymic forms | - |
Schizosaccharomyces pombe NCYC 132S2-2 | - |
2 isozymic forms | - |
Purification (Comment) | Organism |
---|---|
253fold, major isoenzyme form | Schizosaccharomyces pombe |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-hexose = ADP + D-hexose 6-phosphate | equilibrium random Bi Bi mechanism with formation of the abortive enzyme-ADP-glucose complex | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.25 | - |
at pH 7.2 | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Schizosaccharomyces pombe | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | - |
Schizosaccharomyces pombe NCYC 132S2-2 | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway | Schizosaccharomyces pombe | D-glucose 6-phosphate + ADP | - |
? | |
D-glucose + ATP | enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway | Schizosaccharomyces pombe NCYC 132S2-2 | D-glucose 6-phosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47500, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Schizosaccharomyces pombe |