Application | Comment | Organism |
---|---|---|
pharmacology | hexokinase may be an excellent target for the development of a rational chemotherapy against the parasite | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Trypanosoma brucei | rate-limiting step in the glycolysis | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
bloodstream forms, stock 427 | - |
Purification (Comment) | Organism |
---|---|
260fold | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
in purified glycosomes, at 25°C, pH 7.6 | Trypanosoma brucei |
190 | - |
pure enzyme, at 25°C, pH 7.6 | Trypanosoma brucei |
Storage Stability | Organism |
---|---|
4°C, extensive dialysis of purified enzyme against 0.1 M Tris/HCl, pH 7.6, 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Trypanosoma brucei | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | rate-limiting step in the glycolysis | Trypanosoma brucei | D-glucose 6-phosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |