Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
expression in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O-acetylserine | 10 mM, 50% inhibition | Entamoeba histolytica | |
o-phospho-D-serine | 10 mM, 70% inhibition | Entamoeba histolytica | |
O-phospho-L-threonine | 10 mM, 65% inhibition | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0368 | - |
O-phospho-L-serine | pH 8.5, 25°C | Entamoeba histolytica | |
0.043 | - |
2-oxoglutarate | pH 8.5, 25°C | Entamoeba histolytica | |
0.058 | - |
3-phosphohydroxypyruvate | pH 8.5, 25°C | Entamoeba histolytica | |
0.0651 | - |
2-oxoglutarate | pH 8.5, 25°C | Arabidopsis thaliana | |
0.225 | - |
O-phospho-L-serine | pH 8.5, 25°C | Arabidopsis thaliana | |
0.788 | - |
L-glutamate | pH 8.5, 25°C | Entamoeba histolytica | |
0.797 | - |
3-phosphohydroxypyruvate | pH 8.5, 25°C | Arabidopsis thaliana | |
5.05 | - |
L-glutamate | pH 8.5, 25°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Entamoeba histolytica | Q60I38 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
- |
Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.47 | - |
substrate: O-phospho-L-serine, pH 8.5, 25°C | Arabidopsis thaliana |
0.66 | - |
substrate: 2-oxoglutarate, pH 8.5, 25°C | Entamoeba histolytica |
0.67 | - |
substrate: O-phospho-L-serine, pH 8.5, 25°C | Entamoeba histolytica |
0.74 | - |
substrate: 2-oxoglutarate, pH 8.5, 25°C | Arabidopsis thaliana |
5.82 | - |
substrate: L-glutamate, pH 8.5, 25°C | Entamoeba histolytica |
6.14 | - |
substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C | Entamoeba histolytica |
9.65 | - |
substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C | Arabidopsis thaliana |
21.22 | - |
substrate: L-glutamate, pH 8.5, 25°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 3-phosphohydroxypyruvate | - |
Arabidopsis thaliana | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
L-glutamate + 3-phosphohydroxypyruvate | - |
Entamoeba histolytica | O-phospho-L-serine + 2-oxoglutarate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AtPSAT | - |
Arabidopsis thaliana |
EhPSAT | - |
Entamoeba histolytica |
PSAT | - |
Arabidopsis thaliana |
PSAT | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | formation of 3-phosphohydroxypyruvate | Entamoeba histolytica |
8 | 9 | formation of O-phospho-L-serine | Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11 | about 75% of maximal activit at pH 5.5 and pH 11.0, formation of 3-phosphohydroxypyruvate | Entamoeba histolytica |
7 | 10 | about 50% of maximal activity at pH 7.0 and at pH 10.0, formation of O-phospho-L-serine | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | His-tagged recombinant enzyme | - |
8.43 |