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Literature summary for 2.6.1.49 extracted from

  • Nagasaki, T.; Sugita, M.; Fukawa, H.; Lin, H.T.
    DOPA production with Enterobacter cloacae NB 320 by transaminase reaction (1975), Agric. Biol. Chem., 39, 363-369.
No PubMed abstract available
Search for more literature for 2.6.1.49

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 NB 320
-
Enterobacter cloacae NB 320
-
 NB 320
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate aspartate-3,4-dihydroxyphenylpyruvate transaminase, ADT Enterobacter cloacae 3,4-dihydroxy-L-phenylalanine + oxaloacetate i.e. L-dopa ?
3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate aspartate-3,4-dihydroxyphenylpyruvate transaminase, ADT Enterobacter cloacae NB 320 3,4-dihydroxy-L-phenylalanine + oxaloacetate i.e. L-dopa ?
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate glutamate-3,4-dihydroxyphenylpyruvate transaminase, GDT Enterobacter cloacae 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate i.e. L-dopa ?
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate glutamate-3,4-dihydroxyphenylpyruvate transaminase, GDT Enterobacter cloacae NB 320 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate i.e. L-dopa ?
3-(3,4-dihydroxyphenyl)pyruvate + L-phenylalanine phenylalanine-3,4-dihydroxyphenylpyruvate transaminase, PDT Enterobacter cloacae 3,4-dihydroxy-L-phenylalanine + 3-phenylpyruvate i.e. L-dopa ?
3-(3,4-dihydroxyphenyl)pyruvate + L-phenylalanine phenylalanine-3,4-dihydroxyphenylpyruvate transaminase, PDT Enterobacter cloacae NB 320 3,4-dihydroxy-L-phenylalanine + 3-phenylpyruvate i.e. L-dopa ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Enterobacter cloacae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 approx. 40% of maximal activity at 35°C, approx. 90% of maximal activity at 55°C Enterobacter cloacae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 15 min, heat activation, enzyme GDT Enterobacter cloacae
60
-
 15 min, complete loss of activity, enzyme GDT Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 PDT Enterobacter cloacae
8.5
-
 GDT, PDT Enterobacter cloacae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 8.5 45°C, 30 min, enzyme GDT stable Enterobacter cloacae