Protein Variants | Comment | Organism |
---|---|---|
C221S | the mutant shows strongly decreased kcat values compared to the wild type enzyme | Homo sapiens |
C235S | the mutant enzyme exhibits kcat values that are within 20-30% of wild type BCATc values, but also have lower Km values, hence higher calculated kcat/Km values than those of the wild type enzyme | Homo sapiens |
C242S | the mutant shows strongly decreased kcat values compared to the wild type enzyme | Homo sapiens |
C293S | the C293S mutant and wild type BCATc have similar kinetic constants with L-valine, L-leucine, and L-glutamate | Homo sapiens |
C335S | for all amino acid substrates, there is a significant decrease (70% to 80%) in kcat with the C335S mutant, the Km value for glutamate with the C335S mutant enzyme is approximately 3fold lower than that observed with wild type enzyme | Homo sapiens |
C335S/C3388S | the mutant shows kcat values that are similar to values observed with the single C335 mutant enzymes, Km values are largely unchanged compared to those of wild type BCATc, kcat/Km values are significantly lower than wild type values | Homo sapiens |
C338S | the mutant enzyme exhibits kcat values for the BCAAs that were 50% lower than that observed with wild type enzyme. With the exception of L-glutamate, where the Km value is not changed and L-valine, where the Km value is 50% lower than the value for wild type BCATc, Km values for L-isoleucine and L-leucine are largely unaffected for the C338S mutant | Homo sapiens |
General Stability | Organism |
---|---|
on the basis of 5,5'-dithiobis(2-nitrobenzoic acid) titration of wild type BCATc, labeling of 6 thiol groups results in a loss of 60-70% of BCAT activity compared to that of the control enzyme incubated without 5,5'-dithiobis(2-nitrobenzoic acid) and assayed without dithiothreitol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Mono-Q HR 5/5 column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Homo sapiens | 2-oxoisohexanoate + L-glutamate | - |
? | |
L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCATc | cytosolic isoform | Homo sapiens |
branched-chain aminotransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
141 | - |
L-glutamate | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
170 | - |
L-valine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
208 | - |
L-glutamate | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
213 | - |
L-leucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
253 | - |
L-leucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
286 | - |
L-leucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
292 | - |
L-valine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
341 | - |
L-isoleucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
403 | - |
L-glutamate | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
419 | - |
L-isoleucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
430 | - |
L-leucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
443 | - |
L-valine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
503 | - |
L-isoleucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
517 | - |
L-glutamate | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
529 | - |
L-isoleucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
597 | - |
L-valine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
651 | - |
L-leucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
688 | - |
L-valine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
741 | - |
L-glutamate | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
756 | - |
L-leucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
779 | - |
L-isoleucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
803 | - |
L-leucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
838 | - |
L-valine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
843 | - |
L-isoleucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
870 | - |
L-isoleucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
898 | - |
L-leucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1035 | - |
L-glutamate | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1075 | - |
L-glutamate | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1075 | - |
L-isoleucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1111 | - |
L-valine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1157 | - |
L-glutamate | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1236 | - |
L-valine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
L-glutamate | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
31 | - |
L-glutamate | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
40 | - |
L-glutamate | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
43 | - |
L-glutamate | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
62 | - |
L-glutamate | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
89 | - |
L-valine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
103 | - |
L-glutamate | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
108 | - |
L-glutamate | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
126 | - |
L-glutamate | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
132 | - |
L-valine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
201 | - |
L-valine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
313 | - |
L-valine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
474 | - |
L-leucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
505 | - |
L-leucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
579 | - |
L-valine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
597 | - |
L-valine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
618 | - |
L-valine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1059 | - |
L-leucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1118 | - |
L-isoleucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1302 | - |
L-leucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1364 | - |
L-isoleucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1397 | - |
L-valine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1496 | - |
L-isoleucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
1652 | - |
L-leucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
2071 | - |
L-isoleucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
2107 | - |
L-isoleucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
2116 | - |
L-isoleucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
2294 | - |
L-leucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
2494 | - |
L-leucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
3151 | - |
L-leucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
3359 | - |
L-isoleucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
3895 | - |
L-isoleucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens |