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Literature summary for 2.6.1.38 extracted from
- Purification and characterization of L-histidine aminotransferase from nikkomycin-producing Streptomyces tendae Tu901 (1993), J. Gen. Microbiol., 139, 2773-2778.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
pyruvate | pH 8.0, 37°C | Streptomyces tendae |  |
| 25 | - |
L-histidine | pH 8.0, 37°C | Streptomyces tendae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Streptomyces tendae |
| 85000 | - |
gel filtration | Streptomyces tendae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| imidazol-5-yl-pyruvate + L-glutamate | Streptomyces tendae | involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources | histidine + 2-oxoglutarate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces tendae | - |
Tue901/AEC6, mutant hut-11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces tendae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.866 | - |
- |
Streptomyces tendae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| histidine + 2-oxocaproate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-norleucine | - |
r | |
| histidine + 2-oxoglutarate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-glutamate | - |
r | |
| histidine + 2-oxoisovalerate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-valine | - |
r | |
| histidine + 2-oxovalerate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-norvaline | - |
r | |
| imidazol-5-yl-pyruvate + L-glutamate | involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources | Streptomyces tendae | histidine + 2-oxoglutarate | - |
r | |
| L-histidine + 2-oxobutyrate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + 2-aminobutanoate | - |
r | |
| L-histidine + glyoxylate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + glycine | - |
r | |
| L-histidine + pyruvate | ping pong reaction mechanism | Streptomyces tendae | imidazol-5-yl-pyruvate + L-alanine | - |
r | |
| additional information | does not use aromatic amino acids as amino substrates, L-phenylalanine, L-tyrosine, L-tryptophan and D-histidine are no substrates, does not react with L-histidinol phosphate, no activity on gels towards L-alanine, L-leucine, L-lysine, L-glutamate and L-aspartate with pyruvate as amino acceptor, no activity towards L-aspartate with 2-oxoglutarate | Streptomyces tendae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, SDS-PAGE | Streptomyces tendae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HISAT | - |
Streptomyces tendae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptomyces tendae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Streptomyces tendae |
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