Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
2-oxoglutarate | AlaAT1, pH 7.3, 25°C | Triticum aestivum | |
0.22 | - |
pyruvate | AlaAT1, pH 7.3, 25°C | Triticum aestivum | |
0.29 | - |
pyruvate | AlaAT2, pH 7.3, 25°C | Triticum aestivum | |
0.35 | - |
2-oxoglutarate | AlaAT2, pH 7.3, 25°C | Triticum aestivum | |
6.05 | - |
L-alanine | AlaAT1, pH 7.3, 25°C | Triticum aestivum | |
6.25 | - |
L-glutamate | AlaAT1, pH 7.3, 25°C | Triticum aestivum | |
6.66 | - |
L-glutamate | AlaAT2, pH 7.3, 25°C | Triticum aestivum | |
6.9 | - |
L-alanine | AlaAT2, pH 7.3, 25°C | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61300 | - |
SDS-PAGE | Triticum aestivum |
65000 | - |
1 * 65000 | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using saline precipitation, gel filtration, preparative electrophoresis and anion exchange chromatography on protein-Pak Q 8HR column attached to HPLC | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | - |
Triticum aestivum | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Triticum aestivum | pyruvate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000 | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
AlaAT1 | - |
Triticum aestivum |
AlaAT2 | - |
Triticum aestivum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
127.7 | - |
pyruvate | AlaAT1, cosubstrate: L-glutamate, pH 7.3, 25°C | Triticum aestivum | |
127.7 | - |
L-glutamate | AlaAT1, cosubstrate: pyruvate, pH 7.3, 25°C | Triticum aestivum | |
134.9 | - |
L-alanine | AlaAT1, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C | Triticum aestivum | |
134.9 | - |
2-oxoglutarate | AlaAT1, cosubstrate: L-alanine, pH 7.3, 25°C | Triticum aestivum | |
136 | - |
pyruvate | AlaAT2, cosubstrate: L-glutamate, pH 7.3, 25°C | Triticum aestivum | |
136 | - |
L-glutamate | AlaAT2, cosubstrate: pyruvate, pH 7.3, 25°C | Triticum aestivum | |
170 | - |
L-alanine | AlaAT2, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C | Triticum aestivum | |
170 | - |
2-oxoglutarate | AlaAT2, cosubstrate: L-alanine, pH 7.3, 25°C | Triticum aestivum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19630 | - |
pyruvate | AlaAT1, cosubstrate: L-glutamate, pH 7.3, 25°C | Triticum aestivum | |
19630 | - |
L-glutamate | AlaAT1, cosubstrate: pyruvate, pH 7.3, 25°C | Triticum aestivum | |
20510 | - |
pyruvate | AlaAT2, cosubstrate: L-glutamate, pH 7.3, 25°C | Triticum aestivum | |
20510 | - |
L-glutamate | AlaAT2, cosubstrate: pyruvate, pH 7.3, 25°C | Triticum aestivum | |
22290 | - |
L-alanine | AlaAT1, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C | Triticum aestivum | |
22290 | - |
2-oxoglutarate | AlaAT1, cosubstrate: L-alanine, pH 7.3, 25°C | Triticum aestivum | |
24640 | - |
L-alanine | AlaAT2, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C | Triticum aestivum | |
24640 | - |
2-oxoglutarate | AlaAT2, cosubstrate: L-alanine, pH 7.3, 25°C | Triticum aestivum |