Literature summary for 2.6.1.2 extracted from

Son, D.; Jo, J.; Sugiyama, T.
Purification and characterization of alanine aminotransferase from Panicum miliaceum leaves (1991), Arch. Biochem. Biophys., 289, 262-266.

Search for more literature for 2.6.1.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	2-oxoglutarate	pH 7.5, 25°C	Panicum miliaceum
0.33	-	pyruvate	pH 7.5, 25°C	Panicum miliaceum
5	-	glutamate	pH 7.5, 25°C	Panicum miliaceum
6.67	-	alanine	pH 7.5, 25°C	Panicum miliaceum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	2 * 50000, SDS-PAGE	Panicum miliaceum
102000	-	gel filtration	Panicum miliaceum

Organism

Organism	UniProt	Comment	Textmining
Panicum miliaceum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2 minor forms: AlaAT-1, AlaAT-3, 1 major form, AlaAT-2, ammonium sulfate, DEAE-cellulose, Sephacryl S-200, phenyl-Sepharose	Panicum miliaceum

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Panicum miliaceum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
387.9	-	-	Panicum miliaceum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine + 2-oxoglutarate	-	Panicum miliaceum	pyruvate + L-glutamate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 50000, SDS-PAGE	Panicum miliaceum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7.5	forward and reverse reaction	Panicum miliaceum

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Release 2023.1 (January 2023)