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Literature summary for 2.6.1.19 extracted from

  • Liu, W.; Peterson, P.E.; Langston, J.A.; Jin, X.; Zhou, X.; Fisher, A.J.; Toney, M.D.
    Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase (2005), Biochemistry, 44, 2982-2992.
    View publication on PubMed
Search for more literature for 2.6.1.19

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant I50Q, V241A, E211S Escherichia coli

Protein Variants

Protein Variants Comment Organism
E211S crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate Escherichia coli
E211S/I50G drastic decrease in kcat-value Escherichia coli
E211S/I50G/C77K 10fold increase in decarboxylation activity Escherichia coli
E211S/I50G/C77R drastic decrease in kcat-value Escherichia coli
E211S/I50H/V80D 10fold increase in decarboxylation activity, change of reaction specificity to that of a decarboxylase Escherichia coli
E211S/I50H/V80T decrease in kcat, decrease in Km-value for 2-oxoglutarate Escherichia coli
E211S/I50N/V80D drastic decrease in kcat-value Escherichia coli
E211S/I50N/V80T drastic decrease in kcat-value Escherichia coli
E211S/I50Q/G295Y/V241A drastic decrease in kcat-value Escherichia coli
I50Q crystallization data, decrease in kcat, increase in Km-values Escherichia coli
I50Q/G295Y decrease in kcat, decrease in Km-value for 2-oxoglutarate Escherichia coli
V241A crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate substrate inhibition Escherichia coli
Aminooxyacetate
-
 Escherichia coli
Glutarate
-
 Escherichia coli
Maleate
-
 Escherichia coli
additional information no substrate inhibition: 4-aminobutanoate Escherichia coli
succinate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 2-oxoglutarate mutant E211S, 25°C, pH 7.8 Escherichia coli
0.063
-
 2-oxoglutarate mutant E211S/I50H/V80T, 25°C, pH 7.8 Escherichia coli
0.13
-
 2-oxoglutarate mutant V241A, 25°C, pH 7.8 Escherichia coli
0.72
-
 2-oxoglutarate mutant E211S/I50G/C77K, 25°C, pH 7.8 Escherichia coli
0.78
-
 2-oxoglutarate mutant I50Q/G295Y, 25°C, pH 7.8 Escherichia coli
1.07
-
 2-oxoglutarate wild-type, 25°C, pH 7.8 Escherichia coli
4.7
-
 4-aminobutanoate mutant I50Q/G295Y, 25°C, pH 7.8 Escherichia coli
5.8
-
 4-aminobutanoate wild-type, 25°C, pH 7.8 Escherichia coli
5.9
-
 4-aminobutanoate mutant E211S/I50G/C77K, 25°C, pH 7.8 Escherichia coli
30
-
 4-aminobutanoate mutant V241A, 25°C, pH 7.8 Escherichia coli
46
-
 2-oxoglutarate mutant I50Q, 25°C, pH 7.8 Escherichia coli
68
-
 4-aminobutanoate mutant I50Q, 25°C, pH 7.8 Escherichia coli
86
-
 4-aminobutanoate mutant E211S, 25°C, pH 7.8 Escherichia coli
146
-
 4-aminobutanoate mutant E211S/I50H/V80T, 25°C, pH 7.8 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P22256
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate
-
 Escherichia coli 4-oxobutanoate + L-glutamate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00023
-
 4-aminobutanoate mutant E211S/I50H/V80D, 25°C, pH 7.8 Escherichia coli
0.0005
-
 4-aminobutanoate mutant E211S/I50G/C77R, 25°C, pH 7.8 Escherichia coli
0.003
-
 4-aminobutanoate mutant E211S/I50G, 25°C, pH 7.8 Escherichia coli
0.004
-
 4-aminobutanoate mutant E211S/I50Q/G295Y/V241A, 25°C, pH 7.8 Escherichia coli
0.0058
-
 4-aminobutanoate mutant E211S/I50N/V80T, 25°C, pH 7.8 Escherichia coli
0.0075
-
 4-aminobutanoate mutant E211S/I50N/V80D, 25°C, pH 7.8 Escherichia coli
0.028
-
 4-aminobutanoate mutant I50Q/G295Y, 25°C, pH 7.8 Escherichia coli
0.106
-
 4-aminobutanoate mutant E211S/I50H/V80T, 25°C, pH 7.8 Escherichia coli
0.25
-
 4-aminobutanoate mutant E211S/I50G/C77K, 25°C, pH 7.8 Escherichia coli
0.56
-
 4-aminobutanoate mutant E211S, 25°C, pH 7.8 Escherichia coli
1.93
-
 4-aminobutanoate mutant V241A, 25°C, pH 7.8 Escherichia coli
13.5
-
 4-aminobutanoate mutant I50Q, 25°C, pH 7.8 Escherichia coli
47.4
-
 4-aminobutanoate wild-type, 25°C, pH 7.8 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 Aminooxyacetate
-
 Escherichia coli
0.62
-
 Aminooxyacetate wild-type, pH 7.8, 25°C Escherichia coli
1.13
-
 Aminooxyacetate mutant V241A, pH 7.8, 25°C Escherichia coli
3.9
-
 Maleate wild-type, pH 7.8, 25°C Escherichia coli
19
-
 Glutarate wild-type, pH 7.8, 25°C Escherichia coli
27
-
 succinate wild-type, pH 7.8, 25°C Escherichia coli
46
-
 Maleate mutant I50Q, pH 7.8, 25°C Escherichia coli
140
-
 Glutarate mutant V241A, pH 7.8, 25°C Escherichia coli
197
-
 succinate mutant I50Q, pH 7.8, 25°C Escherichia coli
233
-
 Glutarate mutant I50Q, pH 7.8, 25°C Escherichia coli
720
-
 succinate mutant V241A, pH 7.8, 25°C Escherichia coli
780
-
 Maleate mutant V241A, pH 7.8, 25°C Escherichia coli