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Literature summary for 2.6.1.19 extracted from
- 4-Aminobutyrate:2-oxoglutarate aminotransferase from Candida. Purification and properties (1986), Eur. J. Biochem., 156, 589-596.
General Stability
| General Stability | Organism |
|---|---|
| 2-oxoglutarate protects against heat inactivation, better than pyridoxal phosphate | Candida guilliermondii var. membranaefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-aminobutanoate | - |
Candida guilliermondii var. membranaefaciens |  |
| 2-oxoglutarate | - |
Candida guilliermondii var. membranaefaciens | |
| alpha-alanine | - |
Candida guilliermondii var. membranaefaciens | |
| beta-Alanine | - |
Candida guilliermondii var. membranaefaciens | |
| Butyric acid | - |
Candida guilliermondii var. membranaefaciens | |
| propionic acid | - |
Candida guilliermondii var. membranaefaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
2-oxoglutarate | - |
Candida guilliermondii var. membranaefaciens | |
| 2.2 | 2.3 | 4-aminobutanoate | - |
Candida guilliermondii var. membranaefaciens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Candida guilliermondii var. membranaefaciens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
gel filtration | Candida guilliermondii var. membranaefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida guilliermondii var. membranaefaciens | - |
Y43 | - |
| Candida guilliermondii var. membranaefaciens Y43 | - |
Y43 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida guilliermondii var. membranaefaciens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate | mechanism | Candida guilliermondii var. membranaefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52.33 | - |
- |
Candida guilliermondii var. membranaefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate | best substrate | Candida guilliermondii var. membranaefaciens | 4-oxobutanoate + L-glutamate | - |
r | |
| 4-aminobutanoate + 2-oxoglutarate | best substrate | Candida guilliermondii var. membranaefaciens Y43 | 4-oxobutanoate + L-glutamate | - |
r | |
| 5-aminopentanoate + 2-oxoglutarate | transamination at 45% the rate of 4-aminobutanoate | Candida guilliermondii var. membranaefaciens | L-glutamate + 5-oxopentanoate | - |
? | |
| 5-aminopentanoate + 2-oxoglutarate | transamination at 45% the rate of 4-aminobutanoate | Candida guilliermondii var. membranaefaciens Y43 | L-glutamate + 5-oxopentanoate | - |
? | |
| 6-aminohexanoate + 2-oxoglutarate | transamination at 27% the rate of 4-aminobutanoate | Candida guilliermondii var. membranaefaciens | L-glutamate + 6-oxohexanoate | - |
? | |
| 6-aminohexanoate + 2-oxoglutarate | transamination at 27% the rate of 4-aminobutanoate | Candida guilliermondii var. membranaefaciens Y43 | L-glutamate + 6-oxohexanoate | - |
? | |
| beta-alanine + 2-oxoglutarate | poor substrate | Candida guilliermondii var. membranaefaciens | malonic semialdehyde + L-glutamate | - |
r | |
| beta-alanine + 2-oxoglutarate | poor substrate | Candida guilliermondii var. membranaefaciens Y43 | malonic semialdehyde + L-glutamate | - |
r | |
| additional information | no substrates are L-aminovalerate, L-norleucine, straight alpha-amino acids, diamino acids | Candida guilliermondii var. membranaefaciens | ? | - |
? | |
| additional information | no substrates are L-aminovalerate, L-norleucine, straight alpha-amino acids, diamino acids | Candida guilliermondii var. membranaefaciens Y43 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Candida guilliermondii var. membranaefaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | linear increase, above 45°C rapid decrease | Candida guilliermondii var. membranaefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
2-oxoglutarate protects against heat inactivation, better than pyridoxal phosphate | Candida guilliermondii var. membranaefaciens |
| 42 | - |
up to, at least 10 min stable | Candida guilliermondii var. membranaefaciens |
| 46 | - |
up to, at least 10 min stable in the presence of pyridoxal phosphate | Candida guilliermondii var. membranaefaciens |
| 50 | - |
up to, at least 10 min stable in the presence of 2-oxoglutarate | Candida guilliermondii var. membranaefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.7 | Candida guilliermondii var. membranaefaciens |
| 7.8 | 8 | at 35°C | Candida guilliermondii var. membranaefaciens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
Butyric acid | - |
Candida guilliermondii var. membranaefaciens | |
| 0.003 | - |
propionic acid | - |
Candida guilliermondii var. membranaefaciens | |
| 0.033 | - |
2-aminobutanoate | - |
Candida guilliermondii var. membranaefaciens | |
| 0.0355 | - |
beta-Alanine | - |
Candida guilliermondii var. membranaefaciens | |
| 0.039 | - |
alpha-alanine | - |
Candida guilliermondii var. membranaefaciens | |
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