Cloned (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Crystallization (Comment) | Organism |
---|---|
in complex with pyridoxal phosphate and a citrate molecule, to 2.0 A resolution | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
D220A | mutation in pyridoxal phosphate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
K259A | mutation in pyridoxal phosphate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
K41A | mutation in substrate-binding residue, about 60% of wild-type activity | Corynebacterium glutamicum |
N191A | mutation in pyridoxal phosphate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
R144A | mutation in substrate-binding residue, about 25% of wild-type activity | Corynebacterium glutamicum |
R394A | mutation in substrate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
R39A | mutation in substrate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
S103A | mutation in pyridoxal phosphate-binding residue, about 70% of wild-type activity | Corynebacterium glutamicum |
S104A | mutation in pyridoxal phosphate-binding residue, about 70% of wild-type activity | Corynebacterium glutamicum |
S258A | mutation in pyridoxal phosphate-binding residue, about 80% of wild-type activity | Corynebacterium glutamicum |
Y142A | mutation in pyridoxal phosphate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
Y223A | mutation in pyridoxal phosphate-binding residue, almost complete loss of activity | Corynebacterium glutamicum |
Y73A | almost complete loss of activity | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NTR2 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q8NTR2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Corynebacterium glutamicum | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Corynebacterium glutamicum ATCC 13032 | oxaloacetate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cgl0240 | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | tight binding to the enzyme, enzyme exhibits catalytic activity without the addition of pyridoxal phosphate to the reaction mixture. The pyridoxal ring is stabilized by the hydrogen bonds with residues Tyr142, Asn191, Asp220, Tyr223, and Lys259 | Corynebacterium glutamicum |