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Literature summary for 2.6.1.1 extracted from

  • Abendroth, J.; Choi, R.; Wall, A.; Clifton, M.C.; Lukacs, C.M.; Staker, B.L.; Van Voorhis, W.; Myler, P.; Lorimer, D.D.; Edwards, T.E.
    Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia (2015), Acta crystallogr. Sect. F, 71, 566-571.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei brucei
expression in Escherichia coli Leishmania major
expression in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

Crystallization (Comment) Organism
in open an closed conformation. Pyridoxal phosphate is covalently bound to Lys237 via a Schiff base. In mutant K237A, pyridoxal phosphate is clearly bound to the active site Trypanosoma brucei brucei
in open an closed conformation. Pyridoxal phosphate is covalently bound to the active site lysine via a Schiff base Leishmania major
in open an closed conformation. Pyridoxal phosphate is covalently bound to the active site lysine via a Schiff base Giardia intestinalis

Protein Variants

Protein Variants Comment Organism
K237A mutant still binds pyridoxal phosphate even in the absence of the tethering lysine Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Giardia intestinalis A8B1V5
-
-
Giardia intestinalis ATCC 50803 A8B1V5
-
-
Leishmania major Q4FX34
-
-
Trypanosoma brucei brucei Q385Q9
-
-
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 Q385Q9
-
-

Synonyms

Synonyms Comment Organism
AsAT
-
 Leishmania major
GL50803_91056
-
 Giardia intestinalis
Tb11.02.2740
-
 Trypanosoma brucei brucei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate bound to the active site via a Schiff base to a conserved lysine Trypanosoma brucei brucei
pyridoxal 5'-phosphate bound to the active site via a Schiff base to a conserved lysine Leishmania major
pyridoxal 5'-phosphate bound to the active site via a Schiff base to a conserved lysine Giardia intestinalis