Literature summary for 2.6.1.1 extracted from

Kwok, E.Y.; Hanson, M.R.
GFP-labelled Rubisco and aspartate aminotransferase are present in plastid stromules and traffic between plastids (2004), J. Exp. Bot., 55, 595-604.

Cloned(Commentary)

Cloned (Comment)	Organism
aspartate aminotransferase construct consists of the Arabidopsis thaliana ASP5 coding region followed by mGFP5. This construct is designated SP5:GFP. GFP is fused to the carboxy-terminus of ASP5 to preserve the cleavage site between the ASP5 transit peptide and mature protein, thus ensuring proper plastid import and processing. A five amino acid linker peptide (GSGGG) is inserted between ASP5 and GFP to promote proper folding and activity of both proteins. The constructs are introduced into Nicotiana tabacum cv. Samsun NN via Agrobacterium-mediated transformation of leaf strips. ASP5:GFP is present in stromules of Nicotiana tabacum. Arabidopsis thaliana ASP5, with GFP fused to its carboxy-terminus, can interact with itself and with the Nicotiana tabacum AAT3 to form enzymes that are functional in vitro	Arabidopsis thaliana

Cloned (Comment)

Organism

aspartate aminotransferase construct consists of the Arabidopsis thaliana ASP5 coding region followed by mGFP5. This construct is designated SP5:GFP. GFP is fused to the carboxy-terminus of ASP5 to preserve the cleavage site between the ASP5 transit peptide and mature protein, thus ensuring proper plastid import and processing. A five amino acid linker peptide (GSGGG) is inserted between ASP5 and GFP to promote proper folding and activity of both proteins. The constructs are introduced into Nicotiana tabacum cv. Samsun NN via Agrobacterium-mediated transformation of leaf strips. ASP5:GFP is present in stromules of Nicotiana tabacum. Arabidopsis thaliana ASP5, with GFP fused to its carboxy-terminus, can interact with itself and with the Nicotiana tabacum AAT3 to form enzymes that are functional in vitro

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	nuclear-encoded protein that is translated on cytosolic ribosomes and subsequently imported into plastids	Arabidopsis thaliana	9536	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Synonyms

Synonyms	Comment	Organism
AAT3	-	Arabidopsis thaliana
plastid aspartate aminotransferase	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)