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Literature summary for 2.6.1.1 extracted from
- Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotransferase from Arabidopsis thaliana (1998), Protein Expr. Purif., 12, 381-389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| AAT1, AAT2, and AAT5, overexpression in Escherichia coli strains | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| amyloplast | isozyme AAT5 | Arabidopsis thaliana | 9501 | - |
| cytosol | isozyme AAT2 | Arabidopsis thaliana | 5829 | - |
| mitochondrion | isozyme AAT1 | Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | Arabidopsis thaliana | - |
oxaloacetate + L-glutamate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
3 genes encoding 3 different isozymes: asp1 is mitochondrial, asp3 is amyloplastidic and asp2 is cytosolic | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozymes AAT1, AAT2 and AAT5 from Escherichia coli | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | cytosolic, mitochondrial and amyloplastidic isozymes | Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of recombinant isozymes from different E. coli host strains | Arabidopsis thaliana |
| 165 | - |
purified recombinant isozyme AAT5 | Arabidopsis thaliana |
| 454 | - |
recombinant purified isozyme AAT1 | Arabidopsis thaliana |
| 2934 | - |
recombinant purified isozyme AAT2 | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | - |
Arabidopsis thaliana | oxaloacetate + L-glutamate | - |
? | |
| L-aspartate + 2-oxoglutarate | - |
Arabidopsis thaliana | oxaloacetate + L-glutamate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000-45000, isoymes AAT1, AAT2 and AAT5, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 176 | - |
2-oxoglutarate | isozyme AAT5, pH 8.0, 25°C | Arabidopsis thaliana | |
| 205 | - |
2-oxoglutarate | isozyme AAT1, pH 8.0, 25°C | Arabidopsis thaliana | |
| 217 | - |
2-oxoglutarate | isozyme AAT2, pH 8.0, 25°C | Arabidopsis thaliana | |
| 279 | - |
oxaloacetate | isozyme AAT5, pH 8.0, 25°C | Arabidopsis thaliana | |
| 319 | - |
oxaloacetate | isozyme AAT1, pH 8.0, 25°C | Arabidopsis thaliana | |
| 574 | - |
oxaloacetate | isozyme AAT2, pH 8.0, 25°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Arabidopsis thaliana |
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