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Literature summary for 2.6.1.1 extracted from

  • Kim, H.; Ikegami, K.; Nakaoka, M.; Yagi, M.; Shibata, H.; Sawa, Y.
    Characterization of aspartate aminotransferase from the cyanobacterium Phormidium lapideum (2003), Biosci. Biotechnol. Biochem., 67, 490-498.
    View publication on PubMed
Search for more literature for 2.6.1.1

General Stability

General Stability Organism
2-oxoglutarate stabilizes against thermal inactivation Phormidium lapideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 oxaloacetate pH 8.0, 45°C Phormidium lapideum
0.2
-
 2-oxoglutarate pH 8.0, 45°C Phormidium lapideum
5
-
 L-aspartate pH 8.0, 45°C Phormidium lapideum
5.7
-
 L-glutamate pH 8.0, 45°C Phormidium lapideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Phormidium lapideum
85000
-
 gel filtration Phormidium lapideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Phormidium lapideum
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Phormidium lapideum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2450fold Phormidium lapideum

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Phormidium lapideum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
 cell extract Phormidium lapideum
221
-
 purified enzyme Phormidium lapideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxobutyrate 0.4% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + 2-aminobutyrate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Phormidium lapideum oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 2-oxoglutarate is the best acceptor substrate Phormidium lapideum oxaloacetate + L-glutamate
-
 r
L-aspartate + 2-oxoglutarate 45% activity with L-aspartate compared to L-glutamate Phormidium lapideum oxaloacetate + L-glutamate
-
 r
L-aspartate + 2-oxoisocaproate 1.0% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + L-leucine
-
 ?
L-aspartate + phenylpyruvate no activity Phormidium lapideum oxaloacetate + L-phenylalanine
-
 ?
L-aspartate + pyruvate 1.9% activity compared to 2-oxoglutarate Phormidium lapideum oxaloacetate + L-alanine
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate 27% activity compared to L-glutamate Phormidium lapideum 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Phormidium lapideum 2-oxoglutarate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate 0.1% activity compared to L-glutamate Phormidium lapideum L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
additional information substrate specificity Phormidium lapideum ?
-
 ?
additional information indole-3-pyruvate, p-hydroxy-phenylpyruvate, and 2-oxo-n-caproate are no substrates Phormidium lapideum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Phormidium lapideum

Synonyms

Synonyms Comment Organism
AspAT
-
 Phormidium lapideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Phormidium lapideum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 85 44% of maximum activity at 45°C, 80% of maximum activity at 85°C Phormidium lapideum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 2-oxoglutarate protects against thermal inactivation Phormidium lapideum
75
-
-
 Phormidium lapideum
80
-
 10 min, 75% remaining activity Phormidium lapideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
101
-
 L-glutamate pH 8.0, 45°C Phormidium lapideum
187
-
 L-aspartate pH 8.0, 45°C Phormidium lapideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Phormidium lapideum

pH Range

pH Minimum pH Maximum Comment Organism
5 8.8
-
 Phormidium lapideum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Phormidium lapideum
pyridoxal 5'-phosphate enzyme-bound Phormidium lapideum

pI Value

Organism Comment pI Value Maximum pI Value
Phormidium lapideum 2 forms differing in isoelectric point: form I has a pI at pH 4.1, form II at pH 4.2, kinetic mechanism
-
 4.1
Phormidium lapideum 2 forms differing in isoelectric point: form I has a pI at pH 4.1, form II at pH 4.2, kinetic mechanism
-
 4.2