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Literature summary for 2.6.1.1 extracted from

  • Tanaka, T.; Tokuda, T.; Tachibana, A.; Taniguchi, M.; Oi, S.
    Purification and some properties of aspartate aminotransferase of Methanobacterium thermoformicicum SF-4 (1990), Agric. Biol. Chem., 54, 625-631.
No PubMed abstract available
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate competitive to L-aspartate Methanothermobacter thermautotrophicus
adipate moderately Methanothermobacter thermautotrophicus
fumarate weak Methanothermobacter thermautotrophicus
Glutarate weak Methanothermobacter thermautotrophicus
Maleate
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Methanothermobacter thermautotrophicus
57000
-
 gel filtration, gradient PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus SF-4
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus SF-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.44
-
 purified enzyme Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability Organism
-20°C or 4°C, stable for at least 2 weeks Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 25.2% of the activity with glutamate Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 25.2% of the activity with glutamate Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Methanothermobacter thermautotrophicus 2-oxoglutarate + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Methanothermobacter thermautotrophicus SF-4 2-oxoglutarate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate 1.0% of the activity with glutamate Methanothermobacter thermautotrophicus L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
additional information substrate specificity Methanothermobacter thermautotrophicus ?
-
 ?
additional information substrate specificity Methanothermobacter thermautotrophicus SF-4 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 pH 7.5, 1 h, stable up to Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Methanothermobacter thermautotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
7 11
-
 Methanothermobacter thermautotrophicus