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Literature summary for 2.6.1.1 extracted from

  • Lain-Guelbenzu, B.; Munoz-Blanco, J.; Cardenas, J.
    Purification and properties of L-aspartate aminotransferase of Chlamydomonas reinhardtii (1990), Eur. J. Biochem., 188, 529-533.
    View publication on PubMed

General Stability

General Stability Organism
2-oxoglutarate does not protect against heat inactivation Chlamydomonas reinhardtii
addition of glycerol, DTT and pyridoxal 5'-phosphate to buffers, required for stabilization during purification Chlamydomonas reinhardtii
aspartate, 10 mM, or pyridoxal 5'-phosphate, 0.2 mM protects against heat inactivation Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
Chlamydomonas reinhardtii
L-ascorbate
-
Chlamydomonas reinhardtii
Methylacetimidate complete inhibition at 50 mM Chlamydomonas reinhardtii
additional information no substrate inhibition Chlamydomonas reinhardtii
p-hydroxymercuribenzoate
-
Chlamydomonas reinhardtii
Thiosemicarbazide
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
oxaloacetate pH 7.8, 25°C Chlamydomonas reinhardtii
0.55
-
2-oxoglutarate pH 7.8, 25°C Chlamydomonas reinhardtii
2.53
-
L-aspartate pH 7.8, 25°C Chlamydomonas reinhardtii
3.88
-
L-glutamate pH 7.8, 25°C Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
2 * 65000, SDS-PAGE Chlamydomonas reinhardtii
138000
-
gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Chlamydomonas reinhardtii
-
oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate Chlamydomonas reinhardtii 6145c
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii 6145c
-
-
-

Purification (Commentary)

Purification (Comment) Organism
400fold Chlamydomonas reinhardtii

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
purified enzyme Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Chlamydomonas reinhardtii oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate
-
Chlamydomonas reinhardtii 6145c oxaloacetate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate low activity Chlamydomonas reinhardtii 2-oxo-3-phenylpropionic acid + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate low activity Chlamydomonas reinhardtii 6145c 2-oxo-3-phenylpropionic acid + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate low activity Chlamydomonas reinhardtii 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate low activity Chlamydomonas reinhardtii 6145c 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
?
additional information substrate specificity Chlamydomonas reinhardtii ?
-
?
additional information substrate specificity Chlamydomonas reinhardtii 6145c ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 65000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, more than 90% loss of activity Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol of pyridoxal phosphate per mol of enzyme Chlamydomonas reinhardtii
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Chlamydomonas reinhardtii