Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Saccharolobus solfataricus |
53000 | - |
2 * 53000 | Saccharolobus solfataricus |
108000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Saccharolobus solfataricus | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | P14909 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
840fold | Saccharolobus solfataricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Saccharolobus solfataricus |
Renatured (Comment) | Organism |
---|---|
reconstitution of holoenzyme with pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
purified enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Saccharolobus solfataricus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | 48% of the activity with cysteine sulfinic acid | Saccharolobus solfataricus | oxaloacetate + L-glutamate | - |
? | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Saccharolobus solfataricus | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | 25% of the activity with cysteine sulfinic acid | Saccharolobus solfataricus | 2-oxoglutarate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Saccharolobus solfataricus |
dimer | 2 * 53000 | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
95 | - |
above | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 95 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
6 h, stable | Saccharolobus solfataricus |
100 | - |
t1/2: 2 h | Saccharolobus solfataricus |
100 | - |
2 h, 50% inactivation | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | no definite pH optimum | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
irreversible inactivation | Saccharolobus solfataricus |
6 | 9 | stable | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Saccharolobus solfataricus | |
pyridoxal 5'-phosphate | 1 mol of pyridoxal phosphate per mol of subunit | Saccharolobus solfataricus | |
pyridoxal 5'-phosphate | apoenzyme can be reconstituted with pyridoxamine 5phosphate and/or pyridoxal 5'-phosphate, each subunit binding 1 mol of coenzym | Saccharolobus solfataricus | |
pyridoxamine 5'-phosphate | 1 mol per mol of subunit | Saccharolobus solfataricus | |
pyridoxamine 5'-phosphate | reverse reaction | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | microheterogenity of purified enzyme | 6.8 | 6.3 |
Saccharolobus solfataricus | microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed | - |
6.3 |
Saccharolobus solfataricus | microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed | - |
6.8 |