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Literature summary for 2.6.1.1 extracted from

  • Marino, G.; Nitti, G.; Arnone, M.I.; Sannia, G.; Gambacorta, A.; De Rosa, M.
    Purification and characterization of aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1988), J. Biol. Chem., 263, 12305-12309.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE Saccharolobus solfataricus
53000
-
2 * 53000 Saccharolobus solfataricus
108000
-
gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Saccharolobus solfataricus
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P14909
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus
840fold Saccharolobus solfataricus

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Saccharolobus solfataricus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of holoenzyme with pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
purified enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Saccharolobus solfataricus oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate 48% of the activity with cysteine sulfinic acid Saccharolobus solfataricus oxaloacetate + L-glutamate
-
?
L-cysteine sulfinic acid + 2-oxoglutarate
-
Saccharolobus solfataricus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
?
L-glutamate + 2-oxoglutarate 25% of the activity with cysteine sulfinic acid Saccharolobus solfataricus 2-oxoglutarate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Saccharolobus solfataricus
dimer 2 * 53000 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus
95
-
above Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 95
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
6 h, stable Saccharolobus solfataricus
100
-
t1/2: 2 h Saccharolobus solfataricus
100
-
2 h, 50% inactivation Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 no definite pH optimum Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
irreversible inactivation Saccharolobus solfataricus
6 9 stable Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Saccharolobus solfataricus
pyridoxal 5'-phosphate 1 mol of pyridoxal phosphate per mol of subunit Saccharolobus solfataricus
pyridoxal 5'-phosphate apoenzyme can be reconstituted with pyridoxamine 5”phosphate and/or pyridoxal 5'-phosphate, each subunit binding 1 mol of coenzym Saccharolobus solfataricus
pyridoxamine 5'-phosphate 1 mol per mol of subunit Saccharolobus solfataricus
pyridoxamine 5'-phosphate reverse reaction Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus microheterogenity of purified enzyme 6.8 6.3
Saccharolobus solfataricus microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed
-
6.3
Saccharolobus solfataricus microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed
-
6.8