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Literature summary for 2.6.1.1 extracted from

  • Recasens, M.; Benezra, R.; Mandel, P.
    Cysteine sulfinate aminotransferase and aspartate aminotransferase isoenzymes of rat brain. Purification, characterization, and further evidence for identity (1980), Biochemistry, 19, 4583-4589.
    View publication on PubMed
Search for more literature for 2.6.1.1

General Stability

General Stability Organism
cysteinic acid protects the cytosolic isoform against thermal denaturation, not aspartate or 2-oxoglutarate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate competitive to cysteine sulfinic acid or aspartate Rattus norvegicus
gamma-Acetylenic GABA
-
 Rattus norvegicus
L-aspartate weak, competitive against 2-oxoglutarate Rattus norvegicus
L-cysteine sulfinic acid weak, competitive to 2-oxoglutarate Rattus norvegicus
L-cysteinic acid
-
 Rattus norvegicus
L-serine O-sulfate inhibition of cytosolic enzyme, no inhibition of mitochondrial enzyme Rattus norvegicus
additional information no inhibition by dipropylacetic acid, vinyl-GABA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, cytosolic isozyme, SDS-PAGE Rattus norvegicus
43400
-
 2 * 43400, mitochondrial isozyme, SDS-PAGE Rattus norvegicus
84000
-
 cytosolic isozyme, gel filtration Rattus norvegicus
86000
-
 mitochondrial isozyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Rattus norvegicus
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 cytosolic and mitochondrial isozyme
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial and cytosolic isozymes Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
202
-
 purified mitochondrial isozyme Rattus norvegicus
215
-
 purified cytosolic isozyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-diaminobutyric acid + 2-oxoglutarate
-
 Rattus norvegicus ?
-
 ?
2-aminohexanedioic acid + 2-oxoglutarate i.e. alpha-aminoadipic acid Rattus norvegicus 2-oxohexanedioic acid + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate best amino acid donor of cytosolic enzyme Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
L-cysteic acid + 2-oxoglutarate i.e. 2-amino-2-sulfopropionate Rattus norvegicus 2-oxo-3-sulfopropionate + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate best amino acid donor for mitochondrial enzyme Rattus norvegicus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Rattus norvegicus 3-indole-2-oxopropionic acid + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43400, mitochondrial isozyme, SDS-PAGE Rattus norvegicus
dimer 2 * 41000, cytosolic isozyme, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 cysteine sulfinic acid protects against heat inactivation Rattus norvegicus
50
-
 15 min, 10% loss of activity for the mitochondrial isozyme, 80% loss of activity with aspartate for the cytosolic isozyme Rattus norvegicus
70
-
 15 min, mitochondrial enzyme completely destroyed Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10 cytosolic enzyme Rattus norvegicus
8.7
-
 mitochondrial enzyme Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7.4 10 pH 7.4: 60% of activity maximum, pH 8.5-10.0: activity maximum, cytosolic enzyme Rattus norvegicus
7.4 8.7 pH 7.4: 95% of activity maximum, pH 8.7: activity maximum, mitochondrial enzyme Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 irreversibly destroyed below Rattus norvegicus
3.3
-
 1 h, 10% loss of activity of cytoplasmic enzyme, 40% loss of activity of mitochondrial enzyme Rattus norvegicus
12
-
 irreversibly destroyed above Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.96
-
 2-oxoglutarate cytosolic isozyme, pH 8.6, versus cysteinic acid Rattus norvegicus
1.9
-
 2-oxoglutarate mitochondrial isozyme, pH 8.6, versus L-aspartate Rattus norvegicus
2.5
-
 2-oxoglutarate mitochondrial isozyme, pH 8.6, versus cysteinic acid Rattus norvegicus
5.1
-
 L-cysteinic acid cytosolic isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
17
-
 2-oxoglutarate cytosolic isozyme, pH 8.6, versus L-aspartate Rattus norvegicus
21
-
 aspartate cytosolic isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
26
-
 L-cysteinic acid mitochondrial isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
263
-
 aspartate mitochondrial isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus cytoslic isozyme
-
 5.7
Rattus norvegicus mitochondrial isozyme
-
 9.1